Mini-beam modes on standard MX beamline BL17U at SSRF
Wang, QS; Yu, F; Cui, Y; Zhang, KH; Pan, QY; Zhong, CY; Liu, K; Zhou, H; Sun, B; He, JH
2017
发表期刊REVIEW OF SCIENTIFIC INSTRUMENTS
ISSN0034-6748
卷号88期号:7页码:-
文章类型期刊论文
摘要The macromolecular crystallography beamlines at third-generation synchrotron facilities play a central role in solving macromolecular crystal structures and also in understanding the biological function at molecular levels. The MX beamline BL17U at Shanghai Synchrotron Radiation Facility is a typical standard MX beamline with a focused beam size (H x V) of FWHM around 80 mu m x 45 mu m. However the protein samples brought to the beamline are down to 5-10 m from the important and challenging science project now. These samples require smaller size beam. In order to achieve the mini-size beamline, two mini-beam modes have been developed on BL17U: the pinhole-based minibeam and the focused mini-beam by compound refractive lens (CRL). Compared to the pinhole-based mode, three times increase in flux is obtained by the CRL mode at a similar beam size. The flux gain obtained by the CRL needs to be considered for data collection strategies. It takes few minutes to switch the beamline from the normal to CRL mini-beam mode. Published by AIP Publishing.
关键词Macromolecular Crystallography Beamlines Micro-crystallography Protein Crystals Diffraction Instrument Spring-8
DOI10.1063/1.4991682
关键词[WOS]MACROMOLECULAR CRYSTALLOGRAPHY BEAMLINES ; MICRO-CRYSTALLOGRAPHY ; PROTEIN CRYSTALS ; DIFFRACTION ; INSTRUMENT ; SPRING-8
收录类别SCI
语种英语
WOS记录号WOS:000406773700008
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/28736
专题中科院上海应用物理研究所2011-2018年
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Wang, QS,Yu, F,Cui, Y,et al. Mini-beam modes on standard MX beamline BL17U at SSRF[J]. REVIEW OF SCIENTIFIC INSTRUMENTS,2017,88(7):-.
APA Wang, QS.,Yu, F.,Cui, Y.,Zhang, KH.,Pan, QY.,...&He, JH.(2017).Mini-beam modes on standard MX beamline BL17U at SSRF.REVIEW OF SCIENTIFIC INSTRUMENTS,88(7),-.
MLA Wang, QS,et al."Mini-beam modes on standard MX beamline BL17U at SSRF".REVIEW OF SCIENTIFIC INSTRUMENTS 88.7(2017):-.
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