CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
Mini-beam modes on standard MX beamline BL17U at SSRF
Wang, QS; Yu, F; Cui, Y; Zhang, KH; Pan, QY; Zhong, CY; Liu, K; Zhou, H; Sun, B; He, JH
2017
Source PublicationREVIEW OF SCIENTIFIC INSTRUMENTS
ISSN0034-6748
Volume88Issue:7Pages:-
Subtype期刊论文
AbstractThe macromolecular crystallography beamlines at third-generation synchrotron facilities play a central role in solving macromolecular crystal structures and also in understanding the biological function at molecular levels. The MX beamline BL17U at Shanghai Synchrotron Radiation Facility is a typical standard MX beamline with a focused beam size (H x V) of FWHM around 80 mu m x 45 mu m. However the protein samples brought to the beamline are down to 5-10 m from the important and challenging science project now. These samples require smaller size beam. In order to achieve the mini-size beamline, two mini-beam modes have been developed on BL17U: the pinhole-based minibeam and the focused mini-beam by compound refractive lens (CRL). Compared to the pinhole-based mode, three times increase in flux is obtained by the CRL mode at a similar beam size. The flux gain obtained by the CRL needs to be considered for data collection strategies. It takes few minutes to switch the beamline from the normal to CRL mini-beam mode. Published by AIP Publishing.
KeywordMacromolecular Crystallography Beamlines Micro-crystallography Protein Crystals Diffraction Instrument Spring-8
DOI10.1063/1.4991682
WOS KeywordMACROMOLECULAR CRYSTALLOGRAPHY BEAMLINES ; MICRO-CRYSTALLOGRAPHY ; PROTEIN CRYSTALS ; DIFFRACTION ; INSTRUMENT ; SPRING-8
Indexed BySCI
Language英语
WOS IDWOS:000406773700008
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/28736
Collection中科院上海应用物理研究所2011-2018年
Recommended Citation
GB/T 7714
Wang, QS,Yu, F,Cui, Y,et al. Mini-beam modes on standard MX beamline BL17U at SSRF[J]. REVIEW OF SCIENTIFIC INSTRUMENTS,2017,88(7):-.
APA Wang, QS.,Yu, F.,Cui, Y.,Zhang, KH.,Pan, QY.,...&He, JH.(2017).Mini-beam modes on standard MX beamline BL17U at SSRF.REVIEW OF SCIENTIFIC INSTRUMENTS,88(7),-.
MLA Wang, QS,et al."Mini-beam modes on standard MX beamline BL17U at SSRF".REVIEW OF SCIENTIFIC INSTRUMENTS 88.7(2017):-.
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