Nanodiamonds Mediate Oral Delivery of Proteins for Stem Cell Activation and Intestinal Remodeling in Drosophila
Hu, XJ; Li, XJ; Yin, M; Li, P; Huang, P; Wang, LH; Jiang, YG; Wang, H; Chen, N; Fan, CH; Song, HY
2017
发表期刊ACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
卷号9期号:22页码:18575-18583
文章类型期刊论文
摘要Introduction of exogenous biomacromolecules into living systems is of great interest in genome editing, cancer immunotherapy, and stem cell reprogramming. Whereas current strategies generally depend on nucleic acids transfection, direct delivery of functional proteins that provides enhanced specificity, increased safety, and fast and temporal regulation is highly desirable. Nevertheless, intracellular delivery of intact and bioactive proteins, especially in vivo, remains poorly explored. In this study, we developed a nanodiamonds (NDs)-based protein delivery system in cultured cells and in Drosophila that showed high adsorption, high efficiency, and effective cytosolic release of fully functional proteins. Through live-cell imaging, we observed a novel phenomenon wherein a substantial amount of internalized NDsprotein complex rejected fusion with the early endosome, thereby evading protein degradation in the lysosome. More significantly, we demonstrated that dietary NDsRNase induced apoptosis in enterocytes, stimulating regenerative divisions in intestinal stem cells and increasing the number of stem cells and precursor cells in Drosophila intestine. As stem cells are poorly accessible by exogenous agents in vivo, NDs-mediated oral delivery of proteins provides a new approach to modulate the stem cell microenvironment for intestinal remodeling, which has important implications for colorectal cancer therapy and regenerative medicine.
关键词Walled Carbon Nanotubes Fluorescent Nanodiamonds Dna Nanostructures Drug-delivery Nanoparticles Cancer Pathway Autophagy Oxide Biocompatibility
DOI10.1021/acsami.7b04788
关键词[WOS]WALLED CARBON NANOTUBES ; FLUORESCENT NANODIAMONDS ; DNA NANOSTRUCTURES ; DRUG-DELIVERY ; NANOPARTICLES ; CANCER ; PATHWAY ; AUTOPHAGY ; OXIDE ; BIOCOMPATIBILITY
收录类别SCI
语种英语
WOS记录号WOS:000403136400022
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文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/28745
专题中科院上海应用物理研究所2011-2018年
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Hu, XJ,Li, XJ,Yin, M,et al. Nanodiamonds Mediate Oral Delivery of Proteins for Stem Cell Activation and Intestinal Remodeling in Drosophila[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(22):18575-18583.
APA Hu, XJ.,Li, XJ.,Yin, M.,Li, P.,Huang, P.,...&Song, HY.(2017).Nanodiamonds Mediate Oral Delivery of Proteins for Stem Cell Activation and Intestinal Remodeling in Drosophila.ACS APPLIED MATERIALS & INTERFACES,9(22),18575-18583.
MLA Hu, XJ,et al."Nanodiamonds Mediate Oral Delivery of Proteins for Stem Cell Activation and Intestinal Remodeling in Drosophila".ACS APPLIED MATERIALS & INTERFACES 9.22(2017):18575-18583.
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