CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Nuclease-free target recycling signal amplification for ultrasensitive multiplexing DNA biosensing
Zhao, ZH; Chen, SX; Wang, JB; Su, J; Xu, JQ; Mathur, S; Fan, CH; Song, SP
2017
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume94Issue:-Pages:605-608
Subtype期刊论文
AbstractUltrasensitive biosensing technologies without gene amplification held great promise for direct detection of DNA. Herein we report a novel biosensing method, combining target recycling signal-amplification strategy and a homemade electrochemical device. Especially, the target recycling was achieved by a strand displacement process, no needing the help of any nucleases. In the presence of target DNA, the recycling system could be activated to generate a cascade of assembly steps with three hairpin DNA segments. Each recycling process were accompanied by a disassembly step that the last hairpin DNA segment displaces target DNA from the complex at the end of each circulation, freeing targets to activate the self-assembly of more trefoil DNA structures. This biosensing method could detect target DNA at aM level and can distinguish target DNA from interfering DNAs, demonstrating its high sensitivity and high selectivity. Importantly, the biosensing method could work well with serum samples.
KeywordLabel-free Chemiluminescence Detection Strategy Acid
DOI10.1016/j.bios.2017.03.051
Indexed BySCI
WOS KeywordLABEL-FREE ; CHEMILUMINESCENCE DETECTION ; STRATEGY ; ACID
Language英语
WOS IDWOS:000401201300079
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/28754
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Zhao, ZH,Chen, SX,Wang, JB,et al. Nuclease-free target recycling signal amplification for ultrasensitive multiplexing DNA biosensing[J]. BIOSENSORS & BIOELECTRONICS,2017,94(-):605-608.
APA Zhao, ZH.,Chen, SX.,Wang, JB.,Su, J.,Xu, JQ.,...&Song, SP.(2017).Nuclease-free target recycling signal amplification for ultrasensitive multiplexing DNA biosensing.BIOSENSORS & BIOELECTRONICS,94(-),605-608.
MLA Zhao, ZH,et al."Nuclease-free target recycling signal amplification for ultrasensitive multiplexing DNA biosensing".BIOSENSORS & BIOELECTRONICS 94.-(2017):605-608.
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