Nuclease-free target recycling signal amplification for ultrasensitive multiplexing DNA biosensing
Zhao, ZH; Chen, SX; Wang, JB; Su, J; Xu, JQ; Mathur, S; Fan, CH; Song, SP
2017
发表期刊BIOSENSORS & BIOELECTRONICS
ISSN0956-5663
卷号94期号:-页码:605-608
文章类型期刊论文
摘要Ultrasensitive biosensing technologies without gene amplification held great promise for direct detection of DNA. Herein we report a novel biosensing method, combining target recycling signal-amplification strategy and a homemade electrochemical device. Especially, the target recycling was achieved by a strand displacement process, no needing the help of any nucleases. In the presence of target DNA, the recycling system could be activated to generate a cascade of assembly steps with three hairpin DNA segments. Each recycling process were accompanied by a disassembly step that the last hairpin DNA segment displaces target DNA from the complex at the end of each circulation, freeing targets to activate the self-assembly of more trefoil DNA structures. This biosensing method could detect target DNA at aM level and can distinguish target DNA from interfering DNAs, demonstrating its high sensitivity and high selectivity. Importantly, the biosensing method could work well with serum samples.
关键词Label-free Chemiluminescence Detection Strategy Acid
DOI10.1016/j.bios.2017.03.051
关键词[WOS]LABEL-FREE ; CHEMILUMINESCENCE DETECTION ; STRATEGY ; ACID
收录类别SCI
语种英语
WOS记录号WOS:000401201300079
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/28754
专题中科院上海应用物理研究所2011-2018年
推荐引用方式
GB/T 7714
Zhao, ZH,Chen, SX,Wang, JB,et al. Nuclease-free target recycling signal amplification for ultrasensitive multiplexing DNA biosensing[J]. BIOSENSORS & BIOELECTRONICS,2017,94(-):605-608.
APA Zhao, ZH.,Chen, SX.,Wang, JB.,Su, J.,Xu, JQ.,...&Song, SP.(2017).Nuclease-free target recycling signal amplification for ultrasensitive multiplexing DNA biosensing.BIOSENSORS & BIOELECTRONICS,94(-),605-608.
MLA Zhao, ZH,et al."Nuclease-free target recycling signal amplification for ultrasensitive multiplexing DNA biosensing".BIOSENSORS & BIOELECTRONICS 94.-(2017):605-608.
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