PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe
Yang, X; Wen, YL; Wang, LL; Zhou, CQ; Li, Q; Xu, L; Li, LY; Shi, JY; Lal, R; Ren, SZ; Li, J; Jia, NQ; Liu, G
2017
发表期刊ACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
卷号9期号:44页码:38281-38287
文章类型期刊论文
摘要A "sandwich-like" biosensor was developed on the basis of the magnetic bead platform for sensitive detection of breast cancer 1 (BRCA1) DNA. In the present study, a tetrahedron-structured reporter probe (TSRP) was designed, in which 3 vertices of the tetrahedron were labeled with digoxin (Dig), and the other one was labeled with a detection probe. TSRP here provided accurate enzyme loading and well-organized spatial arrangement for optimized signal amplification. The detection limit of this biosensor was as low as 10 fM, which is at least 4 orders of magnitude lower than that of the single DNA probe (100 pM), and the signal gain was 2 times higher than the analysis using three one-dimensional (1D) reporter probes. We could distinguish DNA sequences with only 1 base mismatch, and the performance of our TSRP biosensor was proven to be equally good in both PCR products and real fetal calf serum (FCS) sample as in buffer. We believe this work provided a novel avenue for the development of signal amplification strategies.
关键词Sequence-specific Detection Highly Sensitive Detection Ovarian-cancer Incidence Gold Nanoparticles Electrochemical Detection Signal Amplification Biosensing Interface Protein-detection Magnetic Beads Chain-reaction
DOI10.1021/acsami.7b11994
关键词[WOS]SEQUENCE-SPECIFIC DETECTION ; HIGHLY SENSITIVE DETECTION ; OVARIAN-CANCER INCIDENCE ; GOLD NANOPARTICLES ; ELECTROCHEMICAL DETECTION ; SIGNAL AMPLIFICATION ; BIOSENSING INTERFACE ; PROTEIN-DETECTION ; MAGNETIC BEADS ; CHAIN-REACTION
收录类别SCI
语种英语
WOS记录号WOS:000415140800015
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/28767
专题中科院上海应用物理研究所2011-2018年
推荐引用方式
GB/T 7714
Yang, X,Wen, YL,Wang, LL,et al. PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(44):38281-38287.
APA Yang, X.,Wen, YL.,Wang, LL.,Zhou, CQ.,Li, Q.,...&Liu, G.(2017).PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe.ACS APPLIED MATERIALS & INTERFACES,9(44),38281-38287.
MLA Yang, X,et al."PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe".ACS APPLIED MATERIALS & INTERFACES 9.44(2017):38281-38287.
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