CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe
Yang, X; Wen, YL; Wang, LL; Zhou, CQ; Li, Q; Xu, L; Li, LY; Shi, JY; Lal, R; Ren, SZ; Li, J; Jia, NQ; Liu, G
2017
Source PublicationACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
Volume9Issue:44Pages:38281-38287
Subtype期刊论文
AbstractA "sandwich-like" biosensor was developed on the basis of the magnetic bead platform for sensitive detection of breast cancer 1 (BRCA1) DNA. In the present study, a tetrahedron-structured reporter probe (TSRP) was designed, in which 3 vertices of the tetrahedron were labeled with digoxin (Dig), and the other one was labeled with a detection probe. TSRP here provided accurate enzyme loading and well-organized spatial arrangement for optimized signal amplification. The detection limit of this biosensor was as low as 10 fM, which is at least 4 orders of magnitude lower than that of the single DNA probe (100 pM), and the signal gain was 2 times higher than the analysis using three one-dimensional (1D) reporter probes. We could distinguish DNA sequences with only 1 base mismatch, and the performance of our TSRP biosensor was proven to be equally good in both PCR products and real fetal calf serum (FCS) sample as in buffer. We believe this work provided a novel avenue for the development of signal amplification strategies.
KeywordSequence-specific Detection Highly Sensitive Detection Ovarian-cancer Incidence Gold Nanoparticles Electrochemical Detection Signal Amplification Biosensing Interface Protein-detection Magnetic Beads Chain-reaction
DOI10.1021/acsami.7b11994
WOS KeywordSEQUENCE-SPECIFIC DETECTION ; HIGHLY SENSITIVE DETECTION ; OVARIAN-CANCER INCIDENCE ; GOLD NANOPARTICLES ; ELECTROCHEMICAL DETECTION ; SIGNAL AMPLIFICATION ; BIOSENSING INTERFACE ; PROTEIN-DETECTION ; MAGNETIC BEADS ; CHAIN-REACTION
Indexed BySCI
Language英语
WOS IDWOS:000415140800015
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/28767
Collection中科院上海应用物理研究所2011-2018年
Recommended Citation
GB/T 7714
Yang, X,Wen, YL,Wang, LL,et al. PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(44):38281-38287.
APA Yang, X.,Wen, YL.,Wang, LL.,Zhou, CQ.,Li, Q.,...&Liu, G.(2017).PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe.ACS APPLIED MATERIALS & INTERFACES,9(44),38281-38287.
MLA Yang, X,et al."PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe".ACS APPLIED MATERIALS & INTERFACES 9.44(2017):38281-38287.
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