Real-Time Continuous Identification of Greenhouse Plant Pathogens Based on Recyclable Microfluidic Bioassay System
Qu, XM; Li, M; Zhang, HB; Lin, CL; Wang, F; Xiao, MS; Zhou, Y; Shi, J; Aldalbahi, A; Pei, H; Chen, H; Li, L
2017
发表期刊ACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
卷号9期号:37页码:31568-31575
文章类型期刊论文
摘要The development of a real-time continuous analytical platform for the pathogen detection is of great scientific importance for achieving better disease control and prevention. In this work, we report a rapid and recyclable microfluidic bioassay system constructed from oligonucleotide arrays for selective and sensitive continuous identification of DNA targets of fungal pathogens. We employ the thermal denaturation method to effectively regenerate the oligonucleotide arrays for multiple sample detection, which could considerably reduce the screening effort and costs. The combination of thermal denaturation and laser-induced fluorescence detection technique enables real-time continuous identification of multiple samples (<10 min per sample). As a proof of concept, we have demonstrated that two DNA targets of fungal pathogens (Botrytis cinerea and Didymella bryoniae) can be sequentially analyzed using our rapid microfluidic bioassay system, which provides a new paradigm in the design of microfluidic bioassay system and will be valuable for chemical and biomedical analysis.
关键词Tetrahedral Dna Nanostructures Microarray Analysis Cancer-cells Signal Amplification Gold Nanoparticles Nucleic-acid Chip Microrna Insights Platform
DOI10.1021/acsami.7b10116
关键词[WOS]TETRAHEDRAL DNA NANOSTRUCTURES ; MICROARRAY ANALYSIS ; CANCER-CELLS ; SIGNAL AMPLIFICATION ; GOLD NANOPARTICLES ; NUCLEIC-ACID ; CHIP ; MICRORNA ; INSIGHTS ; PLATFORM
收录类别SCI
语种英语
WOS记录号WOS:000411771400038
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/28794
专题中科院上海应用物理研究所2011-2018年
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GB/T 7714
Qu, XM,Li, M,Zhang, HB,et al. Real-Time Continuous Identification of Greenhouse Plant Pathogens Based on Recyclable Microfluidic Bioassay System[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(37):31568-31575.
APA Qu, XM.,Li, M.,Zhang, HB.,Lin, CL.,Wang, F.,...&Li, L.(2017).Real-Time Continuous Identification of Greenhouse Plant Pathogens Based on Recyclable Microfluidic Bioassay System.ACS APPLIED MATERIALS & INTERFACES,9(37),31568-31575.
MLA Qu, XM,et al."Real-Time Continuous Identification of Greenhouse Plant Pathogens Based on Recyclable Microfluidic Bioassay System".ACS APPLIED MATERIALS & INTERFACES 9.37(2017):31568-31575.
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