CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
Real-Time Continuous Identification of Greenhouse Plant Pathogens Based on Recyclable Microfluidic Bioassay System
Qu, XM; Li, M; Zhang, HB; Lin, CL; Wang, F; Xiao, MS; Zhou, Y; Shi, J; Aldalbahi, A; Pei, H; Chen, H; Li, L
2017
Source PublicationACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
Volume9Issue:37Pages:31568-31575
Subtype期刊论文
AbstractThe development of a real-time continuous analytical platform for the pathogen detection is of great scientific importance for achieving better disease control and prevention. In this work, we report a rapid and recyclable microfluidic bioassay system constructed from oligonucleotide arrays for selective and sensitive continuous identification of DNA targets of fungal pathogens. We employ the thermal denaturation method to effectively regenerate the oligonucleotide arrays for multiple sample detection, which could considerably reduce the screening effort and costs. The combination of thermal denaturation and laser-induced fluorescence detection technique enables real-time continuous identification of multiple samples (<10 min per sample). As a proof of concept, we have demonstrated that two DNA targets of fungal pathogens (Botrytis cinerea and Didymella bryoniae) can be sequentially analyzed using our rapid microfluidic bioassay system, which provides a new paradigm in the design of microfluidic bioassay system and will be valuable for chemical and biomedical analysis.
KeywordTetrahedral Dna Nanostructures Microarray Analysis Cancer-cells Signal Amplification Gold Nanoparticles Nucleic-acid Chip Microrna Insights Platform
DOI10.1021/acsami.7b10116
WOS KeywordTETRAHEDRAL DNA NANOSTRUCTURES ; MICROARRAY ANALYSIS ; CANCER-CELLS ; SIGNAL AMPLIFICATION ; GOLD NANOPARTICLES ; NUCLEIC-ACID ; CHIP ; MICRORNA ; INSIGHTS ; PLATFORM
Indexed BySCI
Language英语
WOS IDWOS:000411771400038
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/28794
Collection中科院上海应用物理研究所2011-2018年
Recommended Citation
GB/T 7714
Qu, XM,Li, M,Zhang, HB,et al. Real-Time Continuous Identification of Greenhouse Plant Pathogens Based on Recyclable Microfluidic Bioassay System[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(37):31568-31575.
APA Qu, XM.,Li, M.,Zhang, HB.,Lin, CL.,Wang, F.,...&Li, L.(2017).Real-Time Continuous Identification of Greenhouse Plant Pathogens Based on Recyclable Microfluidic Bioassay System.ACS APPLIED MATERIALS & INTERFACES,9(37),31568-31575.
MLA Qu, XM,et al."Real-Time Continuous Identification of Greenhouse Plant Pathogens Based on Recyclable Microfluidic Bioassay System".ACS APPLIED MATERIALS & INTERFACES 9.37(2017):31568-31575.
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