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| Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging | |
| Liu, MM; Li, Q; Liang, L; Li, J; Wang, K; Li, JJ; Lv, M; Chen, N; Song, HY; Lee, J; Shi, JY; Wang, LH; Lal, R; Fan, CH | |
| 2017 | |
| Source Publication | NATURE COMMUNICATIONS
 |
| ISSN | 2041-1723 |
| Volume | 8Issue:-Pages:- |
| Subtype | 期刊论文 |
| Abstract | Mechanistic understanding of the endocytosis and intracellular trafficking of nanoparticles is essential for designing smart theranostic carriers. Physico-chemical properties, including size, clustering and surface chemistry of nanoparticles regulate their cellular uptake and transport. Significantly, even single nanoparticles could cluster intracellularly, yet their clustering state and subsequent trafficking are not well understood. Here, we used DNA-decorated gold (fPlas-gold) nanoparticles as a dually emissive fluorescent and plasmonic probe to examine their clustering states and intracellular transport. Evidence from correlative fluorescence and plasmonic imaging shows that endocytosis of fPlas-gold follows multiple pathways. In the early stages of endocytosis, fPlas-gold nanoparticles appear mostly as single particles and they cluster during the vesicular transport and maturation. The speed of encapsulated fPlas-gold transport was critically dependent on the size of clusters but not on the types of organelle such as endosomes and lysosomes. Our results provide key strategies for engineering theranostic nanocarriers for efficient health management. |
| Keyword | Clathrin-mediated Endocytosis Cell Mechanisms Microscopy Endosomes Dynamics Platform Motors Cancer Virus |
| DOI | 10.1038/ncomms15646 |
| Indexed By | SCI |
| WOS Keyword | CLATHRIN-MEDIATED ENDOCYTOSIS ; CELL ; MECHANISMS ; MICROSCOPY ; ENDOSOMES ; DYNAMICS ; PLATFORM ; MOTORS ; CANCER ; VIRUS |
| Language | 英语 |
| WOS ID | WOS:000402395100001 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/28796 |
| Collection | 中科院上海应用物理研究所2011-2019年 |
| Recommended Citation GB/T 7714 | Liu, MM,Li, Q,Liang, L,et al. Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging[J]. NATURE COMMUNICATIONS,2017,8(-):-. |
| APA | Liu, MM.,Li, Q.,Liang, L.,Li, J.,Wang, K.,...&Fan, CH.(2017).Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging.NATURE COMMUNICATIONS,8(-),-. |
| MLA | Liu, MM,et al."Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging".NATURE COMMUNICATIONS 8.-(2017):-. |
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| Real-time visualizat（2920KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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