Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging

doi:10.1038/ncomms15646

CAS OpenIR > 中科院上海应用物理研究所2011-2018年

	Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging
	Liu, MM; Li, Q; Liang, L; Li, J; Wang, K; Li, JJ; Lv, M; Chen, N; Song, HY; Lee, J; Shi, JY; Wang, LH; Lal, R; Fan, CH
	2017
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
卷号	8 期号:-页码:-
文章类型	期刊论文
摘要	Mechanistic understanding of the endocytosis and intracellular trafficking of nanoparticles is essential for designing smart theranostic carriers. Physico-chemical properties, including size, clustering and surface chemistry of nanoparticles regulate their cellular uptake and transport. Significantly, even single nanoparticles could cluster intracellularly, yet their clustering state and subsequent trafficking are not well understood. Here, we used DNA-decorated gold (fPlas-gold) nanoparticles as a dually emissive fluorescent and plasmonic probe to examine their clustering states and intracellular transport. Evidence from correlative fluorescence and plasmonic imaging shows that endocytosis of fPlas-gold follows multiple pathways. In the early stages of endocytosis, fPlas-gold nanoparticles appear mostly as single particles and they cluster during the vesicular transport and maturation. The speed of encapsulated fPlas-gold transport was critically dependent on the size of clusters but not on the types of organelle such as endosomes and lysosomes. Our results provide key strategies for engineering theranostic nanocarriers for efficient health management.
关键词	Clathrin-mediated Endocytosis Cell Mechanisms Microscopy Endosomes Dynamics Platform Motors Cancer Virus
DOI	10.1038/ncomms15646
关键词[WOS]	CLATHRIN-MEDIATED ENDOCYTOSIS ; CELL ; MECHANISMS ; MICROSCOPY ; ENDOSOMES ; DYNAMICS ; PLATFORM ; MOTORS ; CANCER ; VIRUS
收录类别	SCI
语种	英语
WOS记录号	WOS:000402395100001
引用统计
文献类型	期刊论文
条目标识符	http://ir.sinap.ac.cn/handle/331007/28796
专题	中科院上海应用物理研究所2011-2018年
推荐引用方式 GB/T 7714	Liu, MM,Li, Q,Liang, L,et al. Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging[J]. NATURE COMMUNICATIONS,2017,8(-):-.
APA	Liu, MM.,Li, Q.,Liang, L.,Li, J.,Wang, K.,...&Fan, CH.(2017).Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging.NATURE COMMUNICATIONS,8(-),-.
MLA	Liu, MM,et al."Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging".NATURE COMMUNICATIONS 8.-(2017):-.

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