CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
Single copy-sensitive electrochemical assay for circulating methylated DNA in clinical samples with ultrahigh specificity based on a sequential discrimination-amplification strategy
Wang, XY; Chen, F; Zhang, DX; Zhao, Y; Wei, J; Wang, LH; Song, SP; Fan, CH; Zhao, YX
2017
Source PublicationCHEMICAL SCIENCE
ISSN2041-6520
Volume8Issue:7Pages:4764-4770
Subtype期刊论文
AbstractTumor-related circulating methylated DNA represents only a small fraction of the total DNA in clinical samples (e.g. plasma), challenging the accurate analysis of specific DNA methylation patterns. Yet conventional assays based on the real-time quantitative methylation-specific PCR (qMSP) are generally limited in detection sensitivity and specificity due to its non-specific amplification interference including primer dimers and off-target amplification. Here we propose a single copy-sensitive electrochemical assay for circulating methylated DNA with ultrahigh specificity on the basis of a sequential discrimination-amplification strategy. Methylated DNA rather than unmethylated DNA in a bisulfite-modified sample is identified and amplified by the asymmetric MSP to generate abundant biotin-labeled single-stranded amplicons with reduced primer-dimer artifacts. Self-assembled tetrahedral DNA probes, which are readily decorated on an electrode surface as nanostructured probes with ordered orientation and well controlled spacing, enable the highly efficient hybridization of the specific single-stranded amplicons due to greatly increased target accessibility and significantly decreased noise. The interfacial hybridization event is quantitatively translated into electrochemical signals utilizing an enzymatic amplification. The proposed assay integrates dual sequence discrimination processes and cascade signal amplification processes, achieving the identification of as few as one methylated DNA molecule in the presence of a 1000-fold excess of unmethylated alleles. Furthermore, the excellent assay performance enables tumor related methylation detection in lung cancer patients with 200 microlitre plasma samples. The results are in good consistency with those of clinical diagnosis, whereas the conventional qMSP failed to detect the corresponding methylation pattern of these clinically confirmed positive patients in such trace amounts of samples.
KeywordHybridization Chain-reaction High-throughput Assay Promoter Methylation Cancer Probe Sensor Pcr Quantification Resolution Methylight
DOI10.1039/c7sc01035d
WOS KeywordHYBRIDIZATION CHAIN-REACTION ; HIGH-THROUGHPUT ASSAY ; PROMOTER METHYLATION ; CANCER ; PROBE ; SENSOR ; PCR ; QUANTIFICATION ; RESOLUTION ; METHYLIGHT
Indexed BySCI
Language英语
WOS IDWOS:000404617300008
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/28818
Collection中科院上海应用物理研究所2011-2018年
Recommended Citation
GB/T 7714
Wang, XY,Chen, F,Zhang, DX,et al. Single copy-sensitive electrochemical assay for circulating methylated DNA in clinical samples with ultrahigh specificity based on a sequential discrimination-amplification strategy[J]. CHEMICAL SCIENCE,2017,8(7):4764-4770.
APA Wang, XY.,Chen, F.,Zhang, DX.,Zhao, Y.,Wei, J.,...&Zhao, YX.(2017).Single copy-sensitive electrochemical assay for circulating methylated DNA in clinical samples with ultrahigh specificity based on a sequential discrimination-amplification strategy.CHEMICAL SCIENCE,8(7),4764-4770.
MLA Wang, XY,et al."Single copy-sensitive electrochemical assay for circulating methylated DNA in clinical samples with ultrahigh specificity based on a sequential discrimination-amplification strategy".CHEMICAL SCIENCE 8.7(2017):4764-4770.
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