Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization

doi:10.1007/s11426-016-9028-5

CAS OpenIR > 中科院上海应用物理研究所2011-2018年

	Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization
	Gao, ZS; Deng, SH; Li, J; Wang, K; Li, JJ; Wang, LH; Fan, CH
	2017
Source Publication	SCIENCE CHINA-CHEMISTRY
ISSN	1674-7291
Volume	60 Issue:10 Pages:1305-1309
Subtype	期刊论文
Abstract	Stimulated emission depletion (STED) microscope is one of the most prominent super-resolution bio-imaging instruments, which holds great promise for ultrahigh-resolution imaging of cells. To construct a STED microscope, it is challenging to realize temporal synchronization between the excitation pulses and the depletion pulses. In this study, we present a simple and low-cost method to achieve pulse synchronization by using a condensed fluorescent dye as a depletion indicator. By using this method, almost all the confocal microscopes can be upgraded to a STED system without losing its original functions. After the pulse synchronization, our STED system achieved sub-100-nm resolution for fluorescent nanospheres and single-cell imaging.
Keyword	Living Brain-slices Sted Microscopy Fluorescence Microscopy Stimulated-emission Pericentriolar Material Electron-microscopy Nanoscale Light Scattering Reveals
DOI	10.1007/s11426-016-9028-5
WOS Keyword	LIVING BRAIN-SLICES ; STED MICROSCOPY ; FLUORESCENCE MICROSCOPY ; STIMULATED-EMISSION ; PERICENTRIOLAR MATERIAL ; ELECTRON-MICROSCOPY ; NANOSCALE ; LIGHT ; SCATTERING ; REVEALS
Indexed By	SCI
Language	英语
WOS ID	WOS:000415714400006
Citation statistics	Cited Times:8[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.sinap.ac.cn/handle/331007/28836
Collection	中科院上海应用物理研究所2011-2018年
Recommended Citation GB/T 7714	Gao, ZS,Deng, SH,Li, J,et al. Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization[J]. SCIENCE CHINA-CHEMISTRY,2017,60(10):1305-1309.
APA	Gao, ZS.,Deng, SH.,Li, J.,Wang, K.,Li, JJ.,...&Fan, CH.(2017).Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization.SCIENCE CHINA-CHEMISTRY,60(10),1305-1309.
MLA	Gao, ZS,et al."Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization".SCIENCE CHINA-CHEMISTRY 60.10(2017):1305-1309.

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