Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization
Gao, ZS; Deng, SH; Li, J; Wang, K; Li, JJ; Wang, LH; Fan, CH
2017
发表期刊SCIENCE CHINA-CHEMISTRY
ISSN1674-7291
卷号60期号:10页码:1305-1309
文章类型期刊论文
摘要Stimulated emission depletion (STED) microscope is one of the most prominent super-resolution bio-imaging instruments, which holds great promise for ultrahigh-resolution imaging of cells. To construct a STED microscope, it is challenging to realize temporal synchronization between the excitation pulses and the depletion pulses. In this study, we present a simple and low-cost method to achieve pulse synchronization by using a condensed fluorescent dye as a depletion indicator. By using this method, almost all the confocal microscopes can be upgraded to a STED system without losing its original functions. After the pulse synchronization, our STED system achieved sub-100-nm resolution for fluorescent nanospheres and single-cell imaging.
关键词Living Brain-slices Sted Microscopy Fluorescence Microscopy Stimulated-emission Pericentriolar Material Electron-microscopy Nanoscale Light Scattering Reveals
DOI10.1007/s11426-016-9028-5
关键词[WOS]LIVING BRAIN-SLICES ; STED MICROSCOPY ; FLUORESCENCE MICROSCOPY ; STIMULATED-EMISSION ; PERICENTRIOLAR MATERIAL ; ELECTRON-MICROSCOPY ; NANOSCALE ; LIGHT ; SCATTERING ; REVEALS
收录类别SCI
语种英语
WOS记录号WOS:000415714400006
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/28836
专题中科院上海应用物理研究所2011-2018年
推荐引用方式
GB/T 7714
Gao, ZS,Deng, SH,Li, J,et al. Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization[J]. SCIENCE CHINA-CHEMISTRY,2017,60(10):1305-1309.
APA Gao, ZS.,Deng, SH.,Li, J.,Wang, K.,Li, JJ.,...&Fan, CH.(2017).Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization.SCIENCE CHINA-CHEMISTRY,60(10),1305-1309.
MLA Gao, ZS,et al."Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization".SCIENCE CHINA-CHEMISTRY 60.10(2017):1305-1309.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Sub-diffraction-limi(2307KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Gao, ZS]的文章
[Deng, SH]的文章
[Li, J]的文章
百度学术
百度学术中相似的文章
[Gao, ZS]的文章
[Deng, SH]的文章
[Li, J]的文章
必应学术
必应学术中相似的文章
[Gao, ZS]的文章
[Deng, SH]的文章
[Li, J]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。