Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization | |
| Gao, ZS; Deng, SH; Li, J; Wang, K; Li, JJ; Wang, LH; Fan, CH | |
| 2017 | |
| Source Publication | SCIENCE CHINA-CHEMISTRY
 |
| ISSN | 1674-7291 |
| Volume | 60Issue:10Pages:1305-1309 |
| Subtype | 期刊论文 |
| Abstract | Stimulated emission depletion (STED) microscope is one of the most prominent super-resolution bio-imaging instruments, which holds great promise for ultrahigh-resolution imaging of cells. To construct a STED microscope, it is challenging to realize temporal synchronization between the excitation pulses and the depletion pulses. In this study, we present a simple and low-cost method to achieve pulse synchronization by using a condensed fluorescent dye as a depletion indicator. By using this method, almost all the confocal microscopes can be upgraded to a STED system without losing its original functions. After the pulse synchronization, our STED system achieved sub-100-nm resolution for fluorescent nanospheres and single-cell imaging. |
| Keyword | Living Brain-slices Sted Microscopy Fluorescence Microscopy Stimulated-emission Pericentriolar Material Electron-microscopy Nanoscale Light Scattering Reveals |
| DOI | 10.1007/s11426-016-9028-5 |
| Indexed By | SCI |
| WOS Keyword | LIVING BRAIN-SLICES ; STED MICROSCOPY ; FLUORESCENCE MICROSCOPY ; STIMULATED-EMISSION ; PERICENTRIOLAR MATERIAL ; ELECTRON-MICROSCOPY ; NANOSCALE ; LIGHT ; SCATTERING ; REVEALS |
| Language | 英语 |
| WOS ID | WOS:000415714400006 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/28836 |
| Collection | 中科院上海应用物理研究所2011-2020年 |
| Recommended Citation GB/T 7714 | Gao, ZS,Deng, SH,Li, J,et al. Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization[J]. SCIENCE CHINA-CHEMISTRY,2017,60(10):1305-1309. |
| APA | Gao, ZS.,Deng, SH.,Li, J.,Wang, K.,Li, JJ.,...&Fan, CH.(2017).Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization.SCIENCE CHINA-CHEMISTRY,60(10),1305-1309. |
| MLA | Gao, ZS,et al."Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization".SCIENCE CHINA-CHEMISTRY 60.10(2017):1305-1309. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Sub-diffraction-limi（2307KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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