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| An ultrasensitive electrochemical biosensor for the detection of mecA gene in methicillin-resistant Staphylococcus aureus | |
| Xu, L; Liang, W; Wen, YL; Wang, LL; Yang, X; Ren, SZ; Jia, NQ; Zuo, XL; Liu, G | |
| 2018 | |
| 发表期刊 | BIOSENSORS & BIOELECTRONICS
 |
| ISSN | 0956-5663 |
| 卷号 | 99页码:424-430 |
| 文章类型 | 期刊论文 |
| 摘要 | Electrochemical DNA biosensor has unique advantages for on-site pathogenic microorganism detection, yet the detection of long DNA towards genome DNA (gDNA) analysis remains challenge. In this work, we report a novel electrochemical biosensor for the ultrasensitive analysis of mecA DNA on methicillin-resistant Staphylococcus aureus (MRSA) genome, using a multi-signal probes (MSP) system. The MSP consists of 7 biotin-labelled signal probes that will combine to the target DNA in a prehybridization step, and then the complex will be captured by a DNA tetrahedron structure probe (TSP) on the electrode surface. Then, after the introduction of the streptavidin-labelled HRP enzyme, a catalysis current signal is detected that is found to be corresponding to the concentration of the target DNA. MSP in this work plays a critical role not only for the signal amplification through bringing 7 biotins, but also dramatically improves the accessibility of the target sequence embedded in the double-strand DNA molecules and complex second structures. The 3-D DNA TSP here provides steady support and optimized surface density for the very "large" complex of MSP system and gDNA, as a base of the capture probe. Finally, as low as 10 fM synthetic target DNA was successfully detected, which is at least 3 magnitudes lower than that using single signal probe. Most importantly, we demonstrated the practicability of our analysis method by analyzing a 57 fM MRSA gDNA sample showing excellent selectivity, and the reliability of the analysis was also demonstrated by digital PCR. |
| 关键词 | Rolling Circle Amplification Sequence-specific Detection E-dna Sensor Gold Nanoparticles Digital Pcr Mrsa Platform Targets |
| DOI | 10.1016/j.bios.2017.08.014 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000413284200057 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.sinap.ac.cn/handle/331007/28927 |
| 专题 | 中科院上海应用物理研究所2011-2018年 |
| 作者单位 | 1.Xu, L 2.Liang, W 3.Wen, YL 4.Wang, LL 5.Yang, X 6.Ren, SZ 7.Jia, NQ 8.Zuo, XL 9.Liu, G |
| 推荐引用方式 GB/T 7714 | Xu, L,Liang, W,Wen, YL,et al. An ultrasensitive electrochemical biosensor for the detection of mecA gene in methicillin-resistant Staphylococcus aureus[J]. BIOSENSORS & BIOELECTRONICS,2018,99:424-430. |
| APA | Xu, L.,Liang, W.,Wen, YL.,Wang, LL.,Yang, X.,...&Liu, G.(2018).An ultrasensitive electrochemical biosensor for the detection of mecA gene in methicillin-resistant Staphylococcus aureus.BIOSENSORS & BIOELECTRONICS,99,424-430. |
| MLA | Xu, L,et al."An ultrasensitive electrochemical biosensor for the detection of mecA gene in methicillin-resistant Staphylococcus aureus".BIOSENSORS & BIOELECTRONICS 99(2018):424-430. |
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