An ultrasensitive electrochemical biosensor for the detection of mecA gene in methicillin-resistant Staphylococcus aureus
Xu, L; Liang, W; Wen, YL; Wang, LL; Yang, X; Ren, SZ; Jia, NQ; Zuo, XL; Liu, G
2018
发表期刊BIOSENSORS & BIOELECTRONICS
ISSN0956-5663
卷号99页码:424-430
文章类型期刊论文
摘要Electrochemical DNA biosensor has unique advantages for on-site pathogenic microorganism detection, yet the detection of long DNA towards genome DNA (gDNA) analysis remains challenge. In this work, we report a novel electrochemical biosensor for the ultrasensitive analysis of mecA DNA on methicillin-resistant Staphylococcus aureus (MRSA) genome, using a multi-signal probes (MSP) system. The MSP consists of 7 biotin-labelled signal probes that will combine to the target DNA in a prehybridization step, and then the complex will be captured by a DNA tetrahedron structure probe (TSP) on the electrode surface. Then, after the introduction of the streptavidin-labelled HRP enzyme, a catalysis current signal is detected that is found to be corresponding to the concentration of the target DNA. MSP in this work plays a critical role not only for the signal amplification through bringing 7 biotins, but also dramatically improves the accessibility of the target sequence embedded in the double-strand DNA molecules and complex second structures. The 3-D DNA TSP here provides steady support and optimized surface density for the very "large" complex of MSP system and gDNA, as a base of the capture probe. Finally, as low as 10 fM synthetic target DNA was successfully detected, which is at least 3 magnitudes lower than that using single signal probe. Most importantly, we demonstrated the practicability of our analysis method by analyzing a 57 fM MRSA gDNA sample showing excellent selectivity, and the reliability of the analysis was also demonstrated by digital PCR.
关键词Rolling Circle Amplification Sequence-specific Detection E-dna Sensor Gold Nanoparticles Digital Pcr Mrsa Platform Targets
DOI10.1016/j.bios.2017.08.014
收录类别SCI
语种英语
WOS记录号WOS:000413284200057
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/28927
专题中科院上海应用物理研究所2011-2018年
作者单位1.Xu, L
2.Liang, W
3.Wen, YL
4.Wang, LL
5.Yang, X
6.Ren, SZ
7.Jia, NQ
8.Zuo, XL
9.Liu, G
推荐引用方式
GB/T 7714
Xu, L,Liang, W,Wen, YL,et al. An ultrasensitive electrochemical biosensor for the detection of mecA gene in methicillin-resistant Staphylococcus aureus[J]. BIOSENSORS & BIOELECTRONICS,2018,99:424-430.
APA Xu, L.,Liang, W.,Wen, YL.,Wang, LL.,Yang, X.,...&Liu, G.(2018).An ultrasensitive electrochemical biosensor for the detection of mecA gene in methicillin-resistant Staphylococcus aureus.BIOSENSORS & BIOELECTRONICS,99,424-430.
MLA Xu, L,et al."An ultrasensitive electrochemical biosensor for the detection of mecA gene in methicillin-resistant Staphylococcus aureus".BIOSENSORS & BIOELECTRONICS 99(2018):424-430.
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