Bacterial Analysis Using an Electrochemical DNA Biosensor with Poly-Adenine-Mediated DNA Self-Assembly
Li, LY; Wang, LL; Xu, Q; Xu, L; Liang, W; Li, Y; Ding, M; Aldalbahi, A; Ge, ZL; Wang, LH; Yan, J; Lu, N; Li, J; Wen, YL; Liu, G
2018
发表期刊ACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
卷号10期号:8页码:6895-6903
文章类型期刊论文
摘要The spatial arrangement of DNA probes on the electrode surface is of critical significance for the performance probe surface remains challenging. In this work, we develop a of electrochemical biosensors. However, rational control of the capture probe carrying a poly-adenine anchoring block to construct a programmable self-assembled monolayer for a "sandwich-type" electrochemical biosensor. We show that with a co-assembling strategy using a polyA capture probe and 6-mercapto-l-hexanol, the density of the probes on the gold electrode can be simply adjusted by the length of polyA. The electron-transfer effect and thus the hybridization efficiency can as well be optimized by tuning the polyA length. As a result, we obtained an excellent biosensor performance with a limit of detection as low as 5 fM for a synthetic DNA target. We demonstrate the practicability of this system by analyzing a PCR product from Escherichia coli genomic DNA (0.2 pg/mu L). On the basis of the ideal electrochemical interface, our polyA-based biosensor exhibited excellent reusability and stability, which is important for potential applications in the onsite analysis for a wide range of targets.
关键词Sequence-specific Detection Incorporated Nonfouling Surface Single-nucleotide Polymorphism Gold Nanoparticles Label-free Escherichia-coli Pcr Amplicons Stranded-dna Nanostructure Sensor
DOI10.1021/acsami.7b17327
收录类别SCI
语种英语
WOS记录号WOS:000426618000015
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/28933
专题中科院上海应用物理研究所2011-2018年
作者单位1.Li, LY
2.Wang, LL
3.Xu, Q
4.Xu, L
5.Liang, W
6.Li, Y
7.Ding, M
8.Aldalbahi, A
9.Ge, ZL
10.Wang, LH
11.Yan, J
12.Lu, N
13.Li, J
14.Wen, YL
15.Liu, G
推荐引用方式
GB/T 7714
Li, LY,Wang, LL,Xu, Q,et al. Bacterial Analysis Using an Electrochemical DNA Biosensor with Poly-Adenine-Mediated DNA Self-Assembly[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(8):6895-6903.
APA Li, LY.,Wang, LL.,Xu, Q.,Xu, L.,Liang, W.,...&Liu, G.(2018).Bacterial Analysis Using an Electrochemical DNA Biosensor with Poly-Adenine-Mediated DNA Self-Assembly.ACS APPLIED MATERIALS & INTERFACES,10(8),6895-6903.
MLA Li, LY,et al."Bacterial Analysis Using an Electrochemical DNA Biosensor with Poly-Adenine-Mediated DNA Self-Assembly".ACS APPLIED MATERIALS & INTERFACES 10.8(2018):6895-6903.
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