Effective immobilization of Au nanoparticles on TiO2 loaded graphene for a novel sandwich-type immunosensor
Chen, Y; Li, Y; Deng, DM; He, HB; Yan, XX; Wang, ZX; Fan, CH; Luo, LQ
2018
发表期刊BIOSENSORS & BIOELECTRONICS
ISSN0956-5663
卷号102页码:301-306
文章类型期刊论文
摘要A novel and sensitive sandwich immunosensor for amperometric determination of carcinoembryonic antigen (CEA) was designed using Au nanoparticles-titanium dioxide-graphene (AuNPs-TiO2-graphene) nanocomposites. Dopamine-functionalized graphene was firstly prepared by it-stacking interaction, and TiO2 was then attached to the surface of dopamine-graphene by the specificity and high affinity of enediol ligands to Ti (IV). Afterwards, AuNPs-TiO2-graphene nanocomposites were synthesized with photo-reduction approach under ultraviolet irradiation. The morphology and conductivity of the as-prepared nanocomposites were characterized by transmission electron microscopy, Fourier transform infrared spectra, X-ray powder diffraction, cyclic voltammetry and electrochemical impedance spectroscopy. Taking the advantage of large specific surface area and excellent biocompatibility, AuNPs could covalently link horseradish peroxidase labeled secondary antibody (HRP-Ab(2)) through the interaction between AuNPs and mercapto or primary amine groups of HRP-Ab(2) for sandwich-type immunosensor construction. Under optimum conditions, the modified electrode exhibited a linear current response to CEA concentration in a wide range of 0.005-200 ng mL(-1) (R-2 = 0.994) with low detection limit of 3.33 pg mL(-1) (S/N = 3).
关键词Signal Amplification Strategy Selective Nonenzymatic Glucose Electrochemical Immunosensor Carcinoembryonic Antigen Gold Nanoparticles Tio2-graphene Nanocomposites Sensitive Detection Catalyst Electrode Hydrogen-peroxide Tumor-markers
DOI10.1016/j.bios.2017.11.009
收录类别SCI
语种英语
WOS记录号WOS:000424176600040
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/28984
专题中科院上海应用物理研究所2011-2018年
作者单位1.Chen, Y
2.Li, Y
3.Deng, DM
4.He, HB
5.Yan, XX
6.Wang, ZX
7.Fan, CH
8.Luo, LQ
推荐引用方式
GB/T 7714
Chen, Y,Li, Y,Deng, DM,et al. Effective immobilization of Au nanoparticles on TiO2 loaded graphene for a novel sandwich-type immunosensor[J]. BIOSENSORS & BIOELECTRONICS,2018,102:301-306.
APA Chen, Y.,Li, Y.,Deng, DM.,He, HB.,Yan, XX.,...&Luo, LQ.(2018).Effective immobilization of Au nanoparticles on TiO2 loaded graphene for a novel sandwich-type immunosensor.BIOSENSORS & BIOELECTRONICS,102,301-306.
MLA Chen, Y,et al."Effective immobilization of Au nanoparticles on TiO2 loaded graphene for a novel sandwich-type immunosensor".BIOSENSORS & BIOELECTRONICS 102(2018):301-306.
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