Effective immobilization of Au nanoparticles on TiO2 loaded graphene for a novel sandwich-type immunosensor

doi:10.1016/j.bios.2017.11.009

CAS OpenIR > 中科院上海应用物理研究所2011-2019年

	Effective immobilization of Au nanoparticles on TiO2 loaded graphene for a novel sandwich-type immunosensor
	Chen, Y ; Li, Y ; Deng, DM ; He, HB ; Yan, XX ; Wang, ZX ; Fan, CH ; Luo, LQ
	2018
Source Publication	BIOSENSORS & BIOELECTRONICS
ISSN	0956-5663
Volume	102 Pages:301-306
Subtype	期刊论文
Abstract	A novel and sensitive sandwich immunosensor for amperometric determination of carcinoembryonic antigen (CEA) was designed using Au nanoparticles-titanium dioxide-graphene (AuNPs-TiO2-graphene) nanocomposites. Dopamine-functionalized graphene was firstly prepared by it-stacking interaction, and TiO2 was then attached to the surface of dopamine-graphene by the specificity and high affinity of enediol ligands to Ti (IV). Afterwards, AuNPs-TiO2-graphene nanocomposites were synthesized with photo-reduction approach under ultraviolet irradiation. The morphology and conductivity of the as-prepared nanocomposites were characterized by transmission electron microscopy, Fourier transform infrared spectra, X-ray powder diffraction, cyclic voltammetry and electrochemical impedance spectroscopy. Taking the advantage of large specific surface area and excellent biocompatibility, AuNPs could covalently link horseradish peroxidase labeled secondary antibody (HRP-Ab(2)) through the interaction between AuNPs and mercapto or primary amine groups of HRP-Ab(2) for sandwich-type immunosensor construction. Under optimum conditions, the modified electrode exhibited a linear current response to CEA concentration in a wide range of 0.005-200 ng mL(-1) (R-2 = 0.994) with low detection limit of 3.33 pg mL(-1) (S/N = 3).
Keyword	Signal Amplification Strategy Selective Nonenzymatic Glucose Electrochemical Immunosensor Carcinoembryonic Antigen Gold Nanoparticles Tio2-graphene Nanocomposites Sensitive Detection Catalyst Electrode Hydrogen-peroxide Tumor-markers
DOI	10.1016/j.bios.2017.11.009
Indexed By	SCI
Language	英语
WOS ID	WOS:000424176600040
Citation statistics	Cited Times:22[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.sinap.ac.cn/handle/331007/28984
Collection	中科院上海应用物理研究所2011-2019年
Affiliation	1.Chen, Y 2.Li, Y 3.Deng, DM 4.He, HB 5.Yan, XX 6.Wang, ZX 7.Fan, CH 8.Luo, LQ
Recommended Citation GB/T 7714	Chen, Y,Li, Y,Deng, DM,et al. Effective immobilization of Au nanoparticles on TiO2 loaded graphene for a novel sandwich-type immunosensor[J]. BIOSENSORS & BIOELECTRONICS,2018,102:301-306.
APA	Chen, Y.,Li, Y.,Deng, DM.,He, HB.,Yan, XX.,...&Luo, LQ.(2018).Effective immobilization of Au nanoparticles on TiO2 loaded graphene for a novel sandwich-type immunosensor.BIOSENSORS & BIOELECTRONICS,102,301-306.
MLA	Chen, Y,et al."Effective immobilization of Au nanoparticles on TiO2 loaded graphene for a novel sandwich-type immunosensor".BIOSENSORS & BIOELECTRONICS 102(2018):301-306.

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