CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Epitope Binning Assay Using an Electron Transfer-Modulated Aptamer Sensor
Li, M; Guo, XD; Li, H; Zuo, XL; Hao, RZ; Song, HB; Aldalbahi, A; Ge, ZL; Li, J; Li, Q; Song, SP; Li, SH; Shao, NS; Fan, CH; Wang, LH
AbstractSurface plasmon resonance and quartz crystal microbalance are workhorses of protein DNA interaction research for over 20 years, providing ways to quantitatively determine the protein DNA binding. However, the cost, necessary technical expertise, and severe nonspecific adsorption poses barriers to their use. Convenient and effective techniques for the measurement of protein DNA binding affinity and the epitope binning between DNA and proteins for developing highly sensitive detection platform remain challenging. Here, we develop a binding induced alteration in electron transfer kinetics of the redox reporter labeled (methylene blue) on DNA aptamer to measure the binding affinity between prostate-specific antigen (PSA) and aptamer. We demonstrate that the binding of PSA to aptamer decreases the electron transfer rate of methylene blue for similar to 45%. Further, we identify the best pairwise selection of aptamers for developing sandwich assay by sorting from 10 pairwise modes with the PSA detection limit of 500 ng/mL. Our study provides promising ways to analyze the binding affinity between ligand and receptor and to sort pairwise between aptamers or antibodies for the development of highly sensitive sandwich immunoassays.
KeywordSurface-plasmon Resonance Rolling Circle Amplification Quartz-crystal Microbalance Prostate-specific Antigen Signal Amplification Gold Nanoparticles Dna Nanostructure Electrochemical Biosensor Sensitive Detection Binding
Indexed BySCI
WOS IDWOS:000422814400038
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Document Type期刊论文
Affiliation1.Li, M
2.Guo, XD
3.Li, H
4.Zuo, XL
5.Hao, RZ
6.Song, HB
7.Aldalbahi, A
8.Ge, ZL
9.Li, J
10.Li, Q
11.Song, SP
12.Li, SH
13.Shao, NS
14.Fan, CH
15.Wang, LH
Recommended Citation
GB/T 7714
Li, M,Guo, XD,Li, H,et al. Epitope Binning Assay Using an Electron Transfer-Modulated Aptamer Sensor[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(1):341-349.
APA Li, M.,Guo, XD.,Li, H.,Zuo, XL.,Hao, RZ.,...&Wang, LH.(2018).Epitope Binning Assay Using an Electron Transfer-Modulated Aptamer Sensor.ACS APPLIED MATERIALS & INTERFACES,10(1),341-349.
MLA Li, M,et al."Epitope Binning Assay Using an Electron Transfer-Modulated Aptamer Sensor".ACS APPLIED MATERIALS & INTERFACES 10.1(2018):341-349.
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