CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Identifying the Genotypes of Hepatitis B Virus (HBV) with DNA Origami Label
Liu, K; Pan, D; Wen, YQ; Zhang, HL; Chao, J; Wang, LH; Song, SP; Fan, CH; Shi, YY
Source PublicationSMALL
AbstractThe hepatitis B virus (HBV) genotyping may profoundly affect the accurate diagnosis and antiviral treatment of viral hepatitis. Existing genotyping methods such as serological, immunological, or molecular testing are still suffered from substandard specificity and low sensitivity in laboratory or clinical application. In a previous study, a set of high-efficiency hybridizable DNA origami-based shape ID probes to target the templates through which genetic variation could be determined in an ultrahigh resolution of atomic force microscopy (AFM) nanomechanical imaging are established. Here, as a further confirmatory research to explore the sensitivity and applicability of this assay, differentially predesigned DNA origami shape ID probes are also developed for precisely HBV genotyping. Through the specific identification of visualized DNA origami nanostructure with clinical HBV DNA samples, the genetic variation information of genotypes can be directly identified under AFM. As a proof-of-concept, five genotype B and six genotype C are detected in 11 HBV-infected patients' blood DNA samples of Han Chinese population in the single-blinded test. The AFM image-based DNA origami shape ID genotyping approach shows high specificity and sensitivity, which could be promising for virus infection diagnosis and precision medicine in the future.
KeywordAtomic-force Microscopy Surface-antigen Arrays Quantification Quantitation Shapes Probe Field
Indexed BySCI
WOS IDWOS:000424656800002
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Document Type期刊论文
Affiliation1.Liu, K
2.Pan, D
3.Wen, YQ
4.Zhang, HL
5.Chao, J
6.Wang, LH
7.Song, SP
8.Fan, CH
9.Shi, YY
Recommended Citation
GB/T 7714
Liu, K,Pan, D,Wen, YQ,et al. Identifying the Genotypes of Hepatitis B Virus (HBV) with DNA Origami Label[J]. SMALL,2018,14(6):-.
APA Liu, K.,Pan, D.,Wen, YQ.,Zhang, HL.,Chao, J.,...&Shi, YY.(2018).Identifying the Genotypes of Hepatitis B Virus (HBV) with DNA Origami Label.SMALL,14(6),-.
MLA Liu, K,et al."Identifying the Genotypes of Hepatitis B Virus (HBV) with DNA Origami Label".SMALL 14.6(2018):-.
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