Identifying the Genotypes of Hepatitis B Virus (HBV) with DNA Origami Label

doi:10.1002/smll.201701718

CAS OpenIR > 中科院上海应用物理研究所2011-2018年

	Identifying the Genotypes of Hepatitis B Virus (HBV) with DNA Origami Label
	Liu, K ; Pan, D ; Wen, YQ ; Zhang, HL ; Chao, J ; Wang, LH ; Song, SP ; Fan, CH ; Shi, YY
	2018
发表期刊	SMALL
ISSN	1613-6810
卷号	14 期号:6 页码:-
文章类型	期刊论文
摘要	The hepatitis B virus (HBV) genotyping may profoundly affect the accurate diagnosis and antiviral treatment of viral hepatitis. Existing genotyping methods such as serological, immunological, or molecular testing are still suffered from substandard specificity and low sensitivity in laboratory or clinical application. In a previous study, a set of high-efficiency hybridizable DNA origami-based shape ID probes to target the templates through which genetic variation could be determined in an ultrahigh resolution of atomic force microscopy (AFM) nanomechanical imaging are established. Here, as a further confirmatory research to explore the sensitivity and applicability of this assay, differentially predesigned DNA origami shape ID probes are also developed for precisely HBV genotyping. Through the specific identification of visualized DNA origami nanostructure with clinical HBV DNA samples, the genetic variation information of genotypes can be directly identified under AFM. As a proof-of-concept, five genotype B and six genotype C are detected in 11 HBV-infected patients' blood DNA samples of Han Chinese population in the single-blinded test. The AFM image-based DNA origami shape ID genotyping approach shows high specificity and sensitivity, which could be promising for virus infection diagnosis and precision medicine in the future.
关键词	Atomic-force Microscopy Surface-antigen Arrays Quantification Quantitation Shapes Probe Field
DOI	10.1002/smll.201701718
收录类别	SCI
语种	英语
WOS记录号	WOS:000424656800002
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.sinap.ac.cn/handle/331007/29037
专题	中科院上海应用物理研究所2011-2018年
作者单位	1.Liu, K 2.Pan, D 3.Wen, YQ 4.Zhang, HL 5.Chao, J 6.Wang, LH 7.Song, SP 8.Fan, CH 9.Shi, YY
推荐引用方式 GB/T 7714	Liu, K,Pan, D,Wen, YQ,et al. Identifying the Genotypes of Hepatitis B Virus (HBV) with DNA Origami Label[J]. SMALL,2018,14(6):-.
APA	Liu, K.,Pan, D.,Wen, YQ.,Zhang, HL.,Chao, J.,...&Shi, YY.(2018).Identifying the Genotypes of Hepatitis B Virus (HBV) with DNA Origami Label.SMALL,14(6),-.
MLA	Liu, K,et al."Identifying the Genotypes of Hepatitis B Virus (HBV) with DNA Origami Label".SMALL 14.6(2018):-.

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