Identifying the Genotypes of Hepatitis B Virus (HBV) with DNA Origami Label
Liu, K; Pan, D; Wen, YQ; Zhang, HL; Chao, J; Wang, LH; Song, SP; Fan, CH; Shi, YY
2018
发表期刊SMALL
ISSN1613-6810
卷号14期号:6页码:-
文章类型期刊论文
摘要The hepatitis B virus (HBV) genotyping may profoundly affect the accurate diagnosis and antiviral treatment of viral hepatitis. Existing genotyping methods such as serological, immunological, or molecular testing are still suffered from substandard specificity and low sensitivity in laboratory or clinical application. In a previous study, a set of high-efficiency hybridizable DNA origami-based shape ID probes to target the templates through which genetic variation could be determined in an ultrahigh resolution of atomic force microscopy (AFM) nanomechanical imaging are established. Here, as a further confirmatory research to explore the sensitivity and applicability of this assay, differentially predesigned DNA origami shape ID probes are also developed for precisely HBV genotyping. Through the specific identification of visualized DNA origami nanostructure with clinical HBV DNA samples, the genetic variation information of genotypes can be directly identified under AFM. As a proof-of-concept, five genotype B and six genotype C are detected in 11 HBV-infected patients' blood DNA samples of Han Chinese population in the single-blinded test. The AFM image-based DNA origami shape ID genotyping approach shows high specificity and sensitivity, which could be promising for virus infection diagnosis and precision medicine in the future.
关键词Atomic-force Microscopy Surface-antigen Arrays Quantification Quantitation Shapes Probe Field
DOI10.1002/smll.201701718
收录类别SCI
语种英语
WOS记录号WOS:000424656800002
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/29037
专题中科院上海应用物理研究所2011-2018年
作者单位1.Liu, K
2.Pan, D
3.Wen, YQ
4.Zhang, HL
5.Chao, J
6.Wang, LH
7.Song, SP
8.Fan, CH
9.Shi, YY
推荐引用方式
GB/T 7714
Liu, K,Pan, D,Wen, YQ,et al. Identifying the Genotypes of Hepatitis B Virus (HBV) with DNA Origami Label[J]. SMALL,2018,14(6):-.
APA Liu, K.,Pan, D.,Wen, YQ.,Zhang, HL.,Chao, J.,...&Shi, YY.(2018).Identifying the Genotypes of Hepatitis B Virus (HBV) with DNA Origami Label.SMALL,14(6),-.
MLA Liu, K,et al."Identifying the Genotypes of Hepatitis B Virus (HBV) with DNA Origami Label".SMALL 14.6(2018):-.
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