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In Situ Spatial Complementation of Aptamer-Mediated Recognition Enables Live-Cell Imaging of Native RNA Transcripts in Real Time
Wang, ZJ; Luo, Y; Xie, XD; Hu, XJ; Song, HY; Zhao, Y; Shi, JY; Wang, LH; Glinsky, G; Chen, N; Lal, R; Fan, CH
2018
Source PublicationANGEWANDTE CHEMIE-INTERNATIONAL EDITION
ISSN1433-7851
Volume57Issue:4Pages:972-976
Subtype期刊论文
AbstractDirect cellular imaging of the localization and dynamics of biomolecules helps to understand their function and reveals novel mechanisms at the single-cell resolution. In contrast to routine fluorescent-protein-based protein imaging, technology for RNA imaging remains less well explored because of the lack of enabling technology. Herein, we report the development of an aptamer-initiated fluorescence complementation (AiFC) method for RNA imaging by engineering a green fluorescence protein (GFP)-mimicking turn-on RNA aptamer, Broccoli, into two split fragments that could tandemly bind to target mRNA. When genetically encoded in cells, endogenous mRNA molecules recruited Split-Broccoli and brought the two fragments into spatial proximity, which formed a fluorophore-binding site in situ and turned on fluorescence. Significantly, we demonstrated the use of AiFC for high-contrast and real-time imaging of endogenous RNA molecules in living mammalian cells. We envision wide application and practical utility of this enabling technology to in vivo single-cell visualization and mechanistic analysis of macromolecular interactions.
KeywordGreen Fluorescent Protein Messenger-rna Living Cells Spinach Dynamics Reveals Probes Translation Mimics
DOI10.1002/anie.201707795
Indexed BySCI
Language英语
WOS IDWOS:000428208700017
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/29045
Collection中科院上海应用物理研究所2011-2018年
Affiliation1.Wang, ZJ
2.Luo, Y
3.Xie, XD
4.Hu, XJ
5.Song, HY
6.Zhao, Y
7.Shi, JY
8.Wang, LH
9.Glinsky, G
10.Chen, N
11.Lal, R
12.Fan, CH
Recommended Citation
GB/T 7714
Wang, ZJ,Luo, Y,Xie, XD,et al. In Situ Spatial Complementation of Aptamer-Mediated Recognition Enables Live-Cell Imaging of Native RNA Transcripts in Real Time[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2018,57(4):972-976.
APA Wang, ZJ.,Luo, Y.,Xie, XD.,Hu, XJ.,Song, HY.,...&Fan, CH.(2018).In Situ Spatial Complementation of Aptamer-Mediated Recognition Enables Live-Cell Imaging of Native RNA Transcripts in Real Time.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,57(4),972-976.
MLA Wang, ZJ,et al."In Situ Spatial Complementation of Aptamer-Mediated Recognition Enables Live-Cell Imaging of Native RNA Transcripts in Real Time".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 57.4(2018):972-976.
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