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| Lipid-Based Liquid Crystalline Nanoparticles Facilitate Cytosolic Delivery of siRNA via Structural Transformation | |
| He, SF; Fan, WW; Wu, N; Zhu, JJ; Miao, YQ; Miao, XR; Li, FF; Zhang, XX; Gan, Y | |
| 2018 | |
| Source Publication | NANO LETTERS
 |
| ISSN | 1530-6984 |
| Volume | 18Issue:4Pages:2411-2419 |
| Subtype | 期刊论文 |
| Abstract | RNA interference (RNAi) technology has shown great promise for the treatment of cancer and other genetic disorders. Despite the efforts to increase the target tissue distribution, the safe and effective delivery of siRNA to the diseased cells with sufficient cytosolic transport is another critical factor for successful RNAi clinical application. Here, the constructed lipid-based liquid crystalline nanoparticles, called nano-Transformers, can transform the structure in the intracellular acidic environment and perform high-efficient siRNA delivery for cancer treatment. The developed nano-Transformers have satisfactory siRNA loading efficiency and low cytotoxicity. Different from the traditional cationic nanocarriers, the endosomal membrane fusion induced by the conformational transition of lipids contributes to the easy dissociation of siRNA from nanocarriers and direct release of free siRNA into cytoplasm. We show that transfection with cyclin-dependent kinase 1 (CDK1)-siRNA-loaded nano-Transformers causes up to 95% reduction of relevant mRNA in vitro and greatly inhibits the tumor growth without causing any immunogenic response in vivo. This work highlights that the lipid-based nano-Transformers may become the next generation of siRNA delivery system with higher efficacy and improved safety profiles. |
| Keyword | Gene Delivery Drug-delivery Viral Vectors Nonviral Vectors Therapy Therapeutics Nanocarriers Rnai Ph Inhibition |
| DOI | 10.1021/acs.nanolett.7b05430 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000430155900031 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/29061 |
| Collection | 中科院上海应用物理研究所2011-2019年 |
| Affiliation | 1.He, SF 2.Fan, WW 3.Wu, N 4.Zhu, JJ 5.Miao, YQ 6.Miao, XR 7.Li, FF 8.Zhang, XX 9.Gan, Y |
| Recommended Citation GB/T 7714 | He, SF,Fan, WW,Wu, N,et al. Lipid-Based Liquid Crystalline Nanoparticles Facilitate Cytosolic Delivery of siRNA via Structural Transformation[J]. NANO LETTERS,2018,18(4):2411-2419. |
| APA | He, SF.,Fan, WW.,Wu, N.,Zhu, JJ.,Miao, YQ.,...&Gan, Y.(2018).Lipid-Based Liquid Crystalline Nanoparticles Facilitate Cytosolic Delivery of siRNA via Structural Transformation.NANO LETTERS,18(4),2411-2419. |
| MLA | He, SF,et al."Lipid-Based Liquid Crystalline Nanoparticles Facilitate Cytosolic Delivery of siRNA via Structural Transformation".NANO LETTERS 18.4(2018):2411-2419. |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
| Lipid-Based Liquid C（8571KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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