MoS2 Nanoprobe for MicroRNA Quantification Based on Duplex-Specific Nuclease Signal Amplification
Xiao, MS; Man, TT; Zhu, CF; Pei, H; Shi, JY; Li, L; Qu, XM; Shen, XZ; Li, J
2018
发表期刊ACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
卷号10期号:9页码:7852-7858
文章类型期刊论文
摘要MicroRNAs (miRNAs) play significant regulatory roles in physiologic and pathologic processes and are considered as important biomarkers for disease diagnostics and therapeutics. Simple, fast, sensitive, and selective detection of miRNAs, however, is challenged by their short length, low abundance, susceptibility to degradation, and homogenous sequence. Here, we report a novel design of nanoprobes for highly sensitive and selective detection of miRNAs based on MoSrloaded molecular beacons (MBs) and duplex-specific nuclease (DSN)-mediated signal amplification (DSNMSA). We show that MoS, nanosheets not only exhibit high affinity toward MBs but also act as an efficient quencher for absorbed MBs. The strong fluorescence-quenching ability of MoS2 in combination with cyclic DSNMSA contributes to the superior sensitivity of our method, with a limit of detection 4 orders of magnitude lower than that of traditional hybridization methods. Moreover, the nanoprobes also show high selectivity for discriminating homogenous miRNA sequences with one-base differences because of the discrimination ability of MBs and DSN. Furthermore, we demonstrate that the MoS2-loaded MB nanoprobes can be utilized for multiplexed detection of miRNAs. Given its high sensitivity and specificity, as well as the multiplexed function; this novel method as an effective tool shows a great promise for simultaneous quantitative analysis of multiple miRNAs in biomedical research and clinical diagnosis.
关键词Metal Dichalcogenide Nanosheets Dna Nanostructure Molecular Beacons Graphene Oxide Isothermal Amplification Ultrasensitive Detection Ws2 Nanosheet Cancer-cells Fluorescence Identification
DOI10.1021/acsami.7b18984
收录类别SCI
语种英语
WOS记录号WOS:000427204100035
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/29077
专题中科院上海应用物理研究所2011-2018年
作者单位1.Xiao, MS
2.Man, TT
3.Zhu, CF
4.Pei, H
5.Shi, JY
6.Li, L
7.Qu, XM
8.Shen, XZ
9.Li, J
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Xiao, MS,Man, TT,Zhu, CF,et al. MoS2 Nanoprobe for MicroRNA Quantification Based on Duplex-Specific Nuclease Signal Amplification[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(9):7852-7858.
APA Xiao, MS.,Man, TT.,Zhu, CF.,Pei, H.,Shi, JY.,...&Li, J.(2018).MoS2 Nanoprobe for MicroRNA Quantification Based on Duplex-Specific Nuclease Signal Amplification.ACS APPLIED MATERIALS & INTERFACES,10(9),7852-7858.
MLA Xiao, MS,et al."MoS2 Nanoprobe for MicroRNA Quantification Based on Duplex-Specific Nuclease Signal Amplification".ACS APPLIED MATERIALS & INTERFACES 10.9(2018):7852-7858.
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