CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
NanoRNase from Aeropyrum pernix shows nuclease activity on ssDNA and ssRNA
Deng, YJ; Feng, L; Zhou, H; Xiao, X; Wang, FP; Liu, XP
2018
Source PublicationDNA REPAIR
ISSN1568-7864
Volume65Pages:54-63
Subtype期刊论文
AbstractIn cells, degrading DNA and RNA by various nucleases is very important. These processes are strictly controlled and regulated to maintain DNA integrity and to mature or recycle various RNAs. NanoRNase (Nrn) is a 3'exonuclease that specifically degrades nanoRNAs shorter than 5 nucleotides. Several Nrns have been identified and characterized in bacteria, mainly in Firmicutes. Archaea often grow in extreme environments and might be subjected to more damage to DNA/RNA, so DNA repair and recycling of damaged RNA are very important in archaea. There is no report on the identification and characterization of Nrn in archaea. Aeropyrum pernix encodes three potential Nrns: NrnA (Ape1437), NrnB (Ape0124), and an Nrn-like protein Ape2190. Biochemical characterization showed that only Ape0124 could degrade ssDNA and ssRNA from the 3'-end in the presence of Mn2+. Interestingly, unlike bacterial Nrns, Ape0124 prefers ssDNA, including short nanoDNA, and degrades nanoRNA with lower efficiency. The 3'-DNA backbone was found to be required for efficiently hydrolyzing the phosphodiester bonds. In addition, Ape0124 also degrads the 3'-overhang of double-stranded DNA. Interestingly, Ape0124 could hydrolyze pAp into AMP, which is a feature of bacterial NrnA, not NrnB. Our results indicate that Ape0124 is a novel Nrn with a combined substrate profile of bacterial NrnA and NrnB.
KeywordPrime Transcription Initiation Exoribonuclease Rnase-r Escherichia-coli Crystal-structure Messenger-rna Mycobacterium-tuberculosis Bacillus-subtilis Hyperthermophilic Archaea Oligoribonuclease Degradation
DOI10.1016/j.dnarep.2018.03.005
Indexed BySCI
Language英语
WOS IDWOS:000432617300008
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/29080
Collection中科院上海应用物理研究所2011-2018年
Affiliation1.Deng, YJ
2.Feng, L
3.Zhou, H
4.Xiao, X
5.Wang, FP
6.Liu, XP
Recommended Citation
GB/T 7714
Deng, YJ,Feng, L,Zhou, H,et al. NanoRNase from Aeropyrum pernix shows nuclease activity on ssDNA and ssRNA[J]. DNA REPAIR,2018,65:54-63.
APA Deng, YJ,Feng, L,Zhou, H,Xiao, X,Wang, FP,&Liu, XP.(2018).NanoRNase from Aeropyrum pernix shows nuclease activity on ssDNA and ssRNA.DNA REPAIR,65,54-63.
MLA Deng, YJ,et al."NanoRNase from Aeropyrum pernix shows nuclease activity on ssDNA and ssRNA".DNA REPAIR 65(2018):54-63.
Files in This Item: Download All
File Name/Size DocType Version Access License
NanoRNase from Aerop(4668KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Deng, YJ]'s Articles
[Feng, L]'s Articles
[Zhou, H]'s Articles
Baidu academic
Similar articles in Baidu academic
[Deng, YJ]'s Articles
[Feng, L]'s Articles
[Zhou, H]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Deng, YJ]'s Articles
[Feng, L]'s Articles
[Zhou, H]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: NanoRNase from Aeropyrum pernix shows nuclease activity on ssDNA and ssRNA.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.