NanoRNase from Aeropyrum pernix shows nuclease activity on ssDNA and ssRNA
Deng, YJ; Feng, L; Zhou, H; Xiao, X; Wang, FP; Liu, XP
2018
发表期刊DNA REPAIR
ISSN1568-7864
卷号65页码:54-63
文章类型期刊论文
摘要In cells, degrading DNA and RNA by various nucleases is very important. These processes are strictly controlled and regulated to maintain DNA integrity and to mature or recycle various RNAs. NanoRNase (Nrn) is a 3'exonuclease that specifically degrades nanoRNAs shorter than 5 nucleotides. Several Nrns have been identified and characterized in bacteria, mainly in Firmicutes. Archaea often grow in extreme environments and might be subjected to more damage to DNA/RNA, so DNA repair and recycling of damaged RNA are very important in archaea. There is no report on the identification and characterization of Nrn in archaea. Aeropyrum pernix encodes three potential Nrns: NrnA (Ape1437), NrnB (Ape0124), and an Nrn-like protein Ape2190. Biochemical characterization showed that only Ape0124 could degrade ssDNA and ssRNA from the 3'-end in the presence of Mn2+. Interestingly, unlike bacterial Nrns, Ape0124 prefers ssDNA, including short nanoDNA, and degrades nanoRNA with lower efficiency. The 3'-DNA backbone was found to be required for efficiently hydrolyzing the phosphodiester bonds. In addition, Ape0124 also degrads the 3'-overhang of double-stranded DNA. Interestingly, Ape0124 could hydrolyze pAp into AMP, which is a feature of bacterial NrnA, not NrnB. Our results indicate that Ape0124 is a novel Nrn with a combined substrate profile of bacterial NrnA and NrnB.
关键词Prime Transcription Initiation Exoribonuclease Rnase-r Escherichia-coli Crystal-structure Messenger-rna Mycobacterium-tuberculosis Bacillus-subtilis Hyperthermophilic Archaea Oligoribonuclease Degradation
DOI10.1016/j.dnarep.2018.03.005
收录类别SCI
语种英语
WOS记录号WOS:000432617300008
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/29080
专题中科院上海应用物理研究所2011-2018年
作者单位1.Deng, YJ
2.Feng, L
3.Zhou, H
4.Xiao, X
5.Wang, FP
6.Liu, XP
推荐引用方式
GB/T 7714
Deng, YJ,Feng, L,Zhou, H,et al. NanoRNase from Aeropyrum pernix shows nuclease activity on ssDNA and ssRNA[J]. DNA REPAIR,2018,65:54-63.
APA Deng, YJ,Feng, L,Zhou, H,Xiao, X,Wang, FP,&Liu, XP.(2018).NanoRNase from Aeropyrum pernix shows nuclease activity on ssDNA and ssRNA.DNA REPAIR,65,54-63.
MLA Deng, YJ,et al."NanoRNase from Aeropyrum pernix shows nuclease activity on ssDNA and ssRNA".DNA REPAIR 65(2018):54-63.
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