New design for multi-crystal data collection at SSRF
Li, B; Huang, S; Pan, QY; Li, MJ; Zhou, H; Wang, QS; Yu, F; Sun, B; Chen, JQ; He, JH
2018
发表期刊NUCLEAR SCIENCE AND TECHNIQUES
ISSN1001-8042
卷号29期号:2页码:-
文章类型期刊论文
摘要Data collection with microcrystals at synchrotron radiation facilities is challenging because it is difficult to harvest and locate microcrystals. Moreover, microcrystals are sensitive to radiation damage; thus, typically, a complete data set cannot be obtained with a single microcrystal. Herein, we report a new method for data collection with multiple microcrystals having a crystal size ranging from 1 to 30 mu m. This method is suitable for not only low-temperature (100 K) data collection but also room-temperature data collection. Thin Kapton membranes were used to capture multiple crystals simultaneously. The microcrystals were visible under an optical microscope and easily located because the membrane was transparent and sufficiently thin. The film was fixed to a bracket that was prepared using a three-dimensional printer. The bracket was fixed on a magnetic base via screwing and employed by the goniometer system for data collection. Multiple data sets of fatty acid-binding protein 4 (FABP4) and lysozyme microcrystals were collected using this novel designed device. Finally, the structures of protein FABP4 and lysozyme were obtained from these data via the molecule replacement method. The data statistics reveal that this method provides a comparable result to traditional methods such as a nylon loop.
关键词Membrane-proteins Crystallography Radiation Chip
DOI10.1007/s41365-018-0357-5
收录类别SCI
语种英语
WOS记录号WOS:000427326900005
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/29084
专题中科院上海应用物理研究所2011-2018年
作者单位1.Li, B
2.Huang, S
3.Pan, QY
4.Li, MJ
5.Zhou, H
6.Wang, QS
7.Yu, F
8.Sun, B
9.Chen, JQ
10.He, JH
推荐引用方式
GB/T 7714
Li, B,Huang, S,Pan, QY,et al. New design for multi-crystal data collection at SSRF[J]. NUCLEAR SCIENCE AND TECHNIQUES,2018,29(2):-.
APA Li, B.,Huang, S.,Pan, QY.,Li, MJ.,Zhou, H.,...&He, JH.(2018).New design for multi-crystal data collection at SSRF.NUCLEAR SCIENCE AND TECHNIQUES,29(2),-.
MLA Li, B,et al."New design for multi-crystal data collection at SSRF".NUCLEAR SCIENCE AND TECHNIQUES 29.2(2018):-.
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