CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Protein-mimicking nanoparticle (Protmin)-based nanosensor for intracellular analysis of metal ions
Zhu, D; Zhao, DX; Huang, JX; Li, J; Zuo, XL; Wang, LH; Fan, CH
2018
Source PublicationNUCLEAR SCIENCE AND TECHNIQUES
ISSN1001-8042
Volume29Issue:1Pages:-
Subtype期刊论文
AbstractIn this study, we designed and applied protein-mimicking nanoparticles (Protmin) as an intracellular nanosensor for in vivo detection of lead ions (Pb2+). Monodispersed gold nanoparticles (AuNPs) of 13 nm in diameter were modified using poly-adenine-tailed Pb2+ specific 8-17 DNAzyme to form a spherical and functional Protmin. Substrate strands modified with a fluorophore at the 5' end and a quencher at the 3' end were bound to DNAzyme. Pb2+ facilitated cleavage of DNAzyme to release the fluorophore-modified short strands to generate fluorescence. We observed rapid kinetics of the Protmin nanosensor, for which the typical assay time was 10 min. Further, we demonstrated the Protmin nanosensor could readily enter living cells and respond to Pb2+ in the intracellular environment. The broad of range of Protmin designs will be useful for advancing biological and medical applications.
KeywordGold Nanoparticles Living Cells Gene-regulation Lead Ions Dnazyme Oligonucleotides Hybridization Biosensor Catalysis Design
DOI10.1007/s41365-017-0348-y
Indexed BySCI
Language英语
WOS IDWOS:000427326600005
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/29114
Collection中科院上海应用物理研究所2011-2019年
Affiliation1.Zhu, D
2.Zhao, DX
3.Huang, JX
4.Li, J
5.Zuo, XL
6.Wang, LH
7.Fan, CH
Recommended Citation
GB/T 7714
Zhu, D,Zhao, DX,Huang, JX,et al. Protein-mimicking nanoparticle (Protmin)-based nanosensor for intracellular analysis of metal ions[J]. NUCLEAR SCIENCE AND TECHNIQUES,2018,29(1):-.
APA Zhu, D.,Zhao, DX.,Huang, JX.,Li, J.,Zuo, XL.,...&Fan, CH.(2018).Protein-mimicking nanoparticle (Protmin)-based nanosensor for intracellular analysis of metal ions.NUCLEAR SCIENCE AND TECHNIQUES,29(1),-.
MLA Zhu, D,et al."Protein-mimicking nanoparticle (Protmin)-based nanosensor for intracellular analysis of metal ions".NUCLEAR SCIENCE AND TECHNIQUES 29.1(2018):-.
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