Protein-mimicking nanoparticle (Protmin)-based nanosensor for intracellular analysis of metal ions
Zhu, D; Zhao, DX; Huang, JX; Li, J; Zuo, XL; Wang, LH; Fan, CH
2018
发表期刊NUCLEAR SCIENCE AND TECHNIQUES
ISSN1001-8042
卷号29期号:1页码:-
文章类型期刊论文
摘要In this study, we designed and applied protein-mimicking nanoparticles (Protmin) as an intracellular nanosensor for in vivo detection of lead ions (Pb2+). Monodispersed gold nanoparticles (AuNPs) of 13 nm in diameter were modified using poly-adenine-tailed Pb2+ specific 8-17 DNAzyme to form a spherical and functional Protmin. Substrate strands modified with a fluorophore at the 5' end and a quencher at the 3' end were bound to DNAzyme. Pb2+ facilitated cleavage of DNAzyme to release the fluorophore-modified short strands to generate fluorescence. We observed rapid kinetics of the Protmin nanosensor, for which the typical assay time was 10 min. Further, we demonstrated the Protmin nanosensor could readily enter living cells and respond to Pb2+ in the intracellular environment. The broad of range of Protmin designs will be useful for advancing biological and medical applications.
关键词Gold Nanoparticles Living Cells Gene-regulation Lead Ions Dnazyme Oligonucleotides Hybridization Biosensor Catalysis Design
DOI10.1007/s41365-017-0348-y
收录类别SCI
语种英语
WOS记录号WOS:000427326600005
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/29114
专题中科院上海应用物理研究所2011-2018年
作者单位1.Zhu, D
2.Zhao, DX
3.Huang, JX
4.Li, J
5.Zuo, XL
6.Wang, LH
7.Fan, CH
推荐引用方式
GB/T 7714
Zhu, D,Zhao, DX,Huang, JX,et al. Protein-mimicking nanoparticle (Protmin)-based nanosensor for intracellular analysis of metal ions[J]. NUCLEAR SCIENCE AND TECHNIQUES,2018,29(1):-.
APA Zhu, D.,Zhao, DX.,Huang, JX.,Li, J.,Zuo, XL.,...&Fan, CH.(2018).Protein-mimicking nanoparticle (Protmin)-based nanosensor for intracellular analysis of metal ions.NUCLEAR SCIENCE AND TECHNIQUES,29(1),-.
MLA Zhu, D,et al."Protein-mimicking nanoparticle (Protmin)-based nanosensor for intracellular analysis of metal ions".NUCLEAR SCIENCE AND TECHNIQUES 29.1(2018):-.
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