Targeted Imaging of Brain Tumors with a Framework Nucleic Acid Probe

doi:10.1021/acsami.7b17927

CAS OpenIR > 中科院上海应用物理研究所2011-2019年

	Targeted Imaging of Brain Tumors with a Framework Nucleic Acid Probe
	Tian, T ; Li, J ; Xie, C ; Sun, YH ; Lei, HZ ; Liu, XY ; Xia, JY ; Shi, JY ; Wang, LH ; Lu, WY ; Fan, CH
	2018
Source Publication	ACS APPLIED MATERIALS & INTERFACES
ISSN	1944-8244
Volume	10 Issue:4 Pages:3414-3420
Subtype	期刊论文
Abstract	Development of agents for delivering drugs and imaging probes across the blood brain barrier (BBB) remains a major challenge. In this study, we designed a biocompatible framework nucleic acid (FNA)-based imaging probe for brain tumor-targeting. We employed a typical type of FNAs, tetrahedral DNA nanostructures (TDNs), as the building block, which were modified with angiopep-2 (ANG), a 19-mer peptide derived from human Kunitz domain of aprotinin. This probe exhibited high binding efficiency with low-density lipoprotein receptor-related protein-1 (LRP-1) of BBB and glioma. We found that ANG-functionalized TDNs (ANG-TDNs) stayed intact for at least 12 h in serum, and that ANG modification effectively enhanced cellular uptake of TDNs in brain capillary endothelial cells and Uppsala 87 malignant glioma (U87MG) cells. Remarkably, studies in both in vitro and in vivo models revealed that ANG-TDNs could cross the BBB. Especially, in vivo imaging showed strong fluorescent signals in U87MG human glioblastoma xenograft in nude mice. This study establishes that the FNA-based platform provides a new theranostic tool for the study and therapy of brain tumors.
Keyword	Receptor-mediated Transcytosis Dna Nanostructures Delivery Barrier Nanoparticles Angiopep-2 Peptides Origami
DOI	10.1021/acsami.7b17927
Indexed By	SCI
Language	英语
WOS ID	WOS:000424728800030
Citation statistics	Cited Times:22[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.sinap.ac.cn/handle/331007/29166
Collection	中科院上海应用物理研究所2011-2019年
Affiliation	1.Tian, T 2.Li, J 3.Xie, C 4.Sun, YH 5.Lei, HZ 6.Liu, XY 7.Xia, JY 8.Shi, JY 9.Wang, LH 10.Lu, WY 11.Fan, CH
Recommended Citation GB/T 7714	Tian, T,Li, J,Xie, C,et al. Targeted Imaging of Brain Tumors with a Framework Nucleic Acid Probe[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(4):3414-3420.
APA	Tian, T.,Li, J.,Xie, C.,Sun, YH.,Lei, HZ.,...&Fan, CH.(2018).Targeted Imaging of Brain Tumors with a Framework Nucleic Acid Probe.ACS APPLIED MATERIALS & INTERFACES,10(4),3414-3420.
MLA	Tian, T,et al."Targeted Imaging of Brain Tumors with a Framework Nucleic Acid Probe".ACS APPLIED MATERIALS & INTERFACES 10.4(2018):3414-3420.

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