Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells | |
| Yao, SK; Fan, JD; Chen, ZY; Zong, YB; Zhang, JH; Sun, Z; Zhang, L; Tai, R; Liu, Z; Chen, C; Jiang, H | |
| 2018 | |
| Source Publication | IUCRJ
 |
| ISSN | 2052-2525 |
| Volume | 5Pages:141-149 |
| Subtype | 期刊论文 |
| Abstract | Knowledge of the interactions between nanomaterials and large-size mammalian cells, including cellular uptake, intracellular localization and translocation, has greatly advanced nanomedicine and nanotoxicology. Imaging techniques that can locate nanomaterials within the structures of intact large-size cells at nanoscale resolution play crucial roles in acquiring this knowledge. Here, the quantitative imaging of intracellular nanomaterials in three dimensions was performed by combining dual-energy contrast X-ray microscopy and an iterative tomographic algorithm termed equally sloped tomography ( EST). Macrophages with a size of similar to 20 mu m that had been exposed to the potential antitumour agent [Gd@C-82(OH)(22)](n) were investigated. Large numbers of nanoparticles (NPs) aggregated within the cell and were mainly located in phagosomes. No NPs were observed in the nucleus. Imaging of the nanomedicine within whole cells advanced the understanding of the highefficiency antitumour activity and the low toxicity of this agent. This imaging technique can be used to probe nanomaterials within intact large-size cells at nanometre resolution uniformly in three dimensions and may greatly benefit the fields of nanomedicine and nanotoxicology. |
| Keyword | X-ray Tomography Fluorescence Microscopy Tumor Microenvironment Fullerene Derivatives Electron Tomography Nanoparticles Absorption 3d Macrophage Resolution |
| DOI | 10.1107/S2052252517017912 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000427041400006 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/29191 |
| Collection | 中科院上海应用物理研究所2011-2018年 |
| Affiliation | 1.Yao, SK 2.Fan, JD 3.Chen, ZY 4.Zong, YB 5.Zhang, JH 6.Sun, Z 7.Zhang, L 8.Tai, R 9.Liu, Z 10.Chen, C 11.Jiang, H |
| Recommended Citation GB/T 7714 | Yao, SK,Fan, JD,Chen, ZY,et al. Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells[J]. IUCRJ,2018,5:141-149. |
| APA | Yao, SK.,Fan, JD.,Chen, ZY.,Zong, YB.,Zhang, JH.,...&Jiang, H.(2018).Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells.IUCRJ,5,141-149. |
| MLA | Yao, SK,et al."Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells".IUCRJ 5(2018):141-149. |
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| Three-dimensional ul（1709KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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