CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells
Yao, SK; Fan, JD; Chen, ZY; Zong, YB; Zhang, JH; Sun, Z; Zhang, L; Tai, R; Liu, Z; Chen, C; Jiang, H
2018
Source PublicationIUCRJ
ISSN2052-2525
Volume5Pages:141-149
Subtype期刊论文
AbstractKnowledge of the interactions between nanomaterials and large-size mammalian cells, including cellular uptake, intracellular localization and translocation, has greatly advanced nanomedicine and nanotoxicology. Imaging techniques that can locate nanomaterials within the structures of intact large-size cells at nanoscale resolution play crucial roles in acquiring this knowledge. Here, the quantitative imaging of intracellular nanomaterials in three dimensions was performed by combining dual-energy contrast X-ray microscopy and an iterative tomographic algorithm termed equally sloped tomography ( EST). Macrophages with a size of similar to 20 mu m that had been exposed to the potential antitumour agent [Gd@C-82(OH)(22)](n) were investigated. Large numbers of nanoparticles (NPs) aggregated within the cell and were mainly located in phagosomes. No NPs were observed in the nucleus. Imaging of the nanomedicine within whole cells advanced the understanding of the highefficiency antitumour activity and the low toxicity of this agent. This imaging technique can be used to probe nanomaterials within intact large-size cells at nanometre resolution uniformly in three dimensions and may greatly benefit the fields of nanomedicine and nanotoxicology.
KeywordX-ray Tomography Fluorescence Microscopy Tumor Microenvironment Fullerene Derivatives Electron Tomography Nanoparticles Absorption 3d Macrophage Resolution
DOI10.1107/S2052252517017912
Indexed BySCI
Language英语
WOS IDWOS:000427041400006
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/29191
Collection中科院上海应用物理研究所2011-2018年
Affiliation1.Yao, SK
2.Fan, JD
3.Chen, ZY
4.Zong, YB
5.Zhang, JH
6.Sun, Z
7.Zhang, L
8.Tai, R
9.Liu, Z
10.Chen, C
11.Jiang, H
Recommended Citation
GB/T 7714
Yao, SK,Fan, JD,Chen, ZY,et al. Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells[J]. IUCRJ,2018,5:141-149.
APA Yao, SK.,Fan, JD.,Chen, ZY.,Zong, YB.,Zhang, JH.,...&Jiang, H.(2018).Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells.IUCRJ,5,141-149.
MLA Yao, SK,et al."Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells".IUCRJ 5(2018):141-149.
Files in This Item: Download All
File Name/Size DocType Version Access License
Three-dimensional ul(1709KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yao, SK]'s Articles
[Fan, JD]'s Articles
[Chen, ZY]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yao, SK]'s Articles
[Fan, JD]'s Articles
[Chen, ZY]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yao, SK]'s Articles
[Fan, JD]'s Articles
[Chen, ZY]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.