Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells
Yao, SK; Fan, JD; Chen, ZY; Zong, YB; Zhang, JH; Sun, Z; Zhang, L; Tai, R; Liu, Z; Chen, C; Jiang, H
2018
发表期刊IUCRJ
ISSN2052-2525
卷号5页码:141-149
文章类型期刊论文
摘要Knowledge of the interactions between nanomaterials and large-size mammalian cells, including cellular uptake, intracellular localization and translocation, has greatly advanced nanomedicine and nanotoxicology. Imaging techniques that can locate nanomaterials within the structures of intact large-size cells at nanoscale resolution play crucial roles in acquiring this knowledge. Here, the quantitative imaging of intracellular nanomaterials in three dimensions was performed by combining dual-energy contrast X-ray microscopy and an iterative tomographic algorithm termed equally sloped tomography ( EST). Macrophages with a size of similar to 20 mu m that had been exposed to the potential antitumour agent [Gd@C-82(OH)(22)](n) were investigated. Large numbers of nanoparticles (NPs) aggregated within the cell and were mainly located in phagosomes. No NPs were observed in the nucleus. Imaging of the nanomedicine within whole cells advanced the understanding of the highefficiency antitumour activity and the low toxicity of this agent. This imaging technique can be used to probe nanomaterials within intact large-size cells at nanometre resolution uniformly in three dimensions and may greatly benefit the fields of nanomedicine and nanotoxicology.
关键词X-ray Tomography Fluorescence Microscopy Tumor Microenvironment Fullerene Derivatives Electron Tomography Nanoparticles Absorption 3d Macrophage Resolution
DOI10.1107/S2052252517017912
收录类别SCI
语种英语
WOS记录号WOS:000427041400006
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/29191
专题中科院上海应用物理研究所2011-2018年
作者单位1.Yao, SK
2.Fan, JD
3.Chen, ZY
4.Zong, YB
5.Zhang, JH
6.Sun, Z
7.Zhang, L
8.Tai, R
9.Liu, Z
10.Chen, C
11.Jiang, H
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GB/T 7714
Yao, SK,Fan, JD,Chen, ZY,et al. Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells[J]. IUCRJ,2018,5:141-149.
APA Yao, SK.,Fan, JD.,Chen, ZY.,Zong, YB.,Zhang, JH.,...&Jiang, H.(2018).Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells.IUCRJ,5,141-149.
MLA Yao, SK,et al."Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells".IUCRJ 5(2018):141-149.
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