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Multiple Amplified Electrochemical Detection of MicroRNA-21 Using Hierarchical Flower-like Gold Nanostructures Combined with Gold-enriched Hybridization Chain Reaction
Zhu, D; Liu, W; Cao, WF; Chao, J; Su, S; Wang, LH; Fan, CH
2018
Source PublicationELECTROANALYSIS
ISSN1040-0397
Volume30Issue:7Pages:1349-1356
Subtype期刊论文
AbstractIn this work, we present a multiple amplified electrochemical method that can detect target microRNA-21 (miRNA-21) with high sensitivity by using hierarchical flower-like gold nanostructures (HFGNs) and gold-enriched enzyme-free amplification. The HFGNs deposited on ITO conducting glass served as the electrode substrate with large specific surface area, which allowed the immobilization of large amounts of capture DNA (DNA-1). Then, the gold-enriched hybridization chain reaction (HCR) for signal amplification was attached onto the electrode through target miRNA-mediated sandwich hybridization. The initial strands (I-DNA) enriched on gold nanoparticles (AuNPs) provided plenty of sites for the triggering of the HCR reaction after the addition of H1 and H2 strands, which provided exponential increase of binding sites for the loading of electrochemical signal molecules [Ru(NH3)(6)](3+). Therefore, multiple signal amplification could be achieved to reach an ultrasensitive detection of miRNA-21. A wide linear dynamic range from 1fM to 1nM and a detection limit of 0.12fM (S/N=3) was reached with good selectivity. The electrochemical assay method in this work may hold a great potential for clinical diagnosis of genetic disease in the future.
KeywordDNA NANOSTRUCTURE REACTION AMPLIFICATION CANCER GRAPHENE NANOPARTICLES EXPRESSION BIOSENSORS PLATFORM CELLS
DOI10.1002/elan.201700696
Indexed BySCI
Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/31038
Collection中科院上海应用物理研究所2011-2020年
Affiliation1.Nanjing Univ Posts & Telecommun, Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Inst Adv Mat, Key Lab Organ Elect & Informat Displays, 9 Wenyuan Rd, Nanjing 210023, Jiangsu, Peoples R China
2.Nanjing Univ Posts & Telecommun, Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Inst Adv Mat, Jiangsu Key Lab Biosensors, 9 Wenyuan Rd, Nanjing 210023, Jiangsu, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol, Shanghai 201800, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Bioimaging Ctr, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Zhu, D,Liu, W,Cao, WF,et al. Multiple Amplified Electrochemical Detection of MicroRNA-21 Using Hierarchical Flower-like Gold Nanostructures Combined with Gold-enriched Hybridization Chain Reaction[J]. ELECTROANALYSIS,2018,30(7):1349-1356.
APA Zhu, D.,Liu, W.,Cao, WF.,Chao, J.,Su, S.,...&Fan, CH.(2018).Multiple Amplified Electrochemical Detection of MicroRNA-21 Using Hierarchical Flower-like Gold Nanostructures Combined with Gold-enriched Hybridization Chain Reaction.ELECTROANALYSIS,30(7),1349-1356.
MLA Zhu, D,et al."Multiple Amplified Electrochemical Detection of MicroRNA-21 Using Hierarchical Flower-like Gold Nanostructures Combined with Gold-enriched Hybridization Chain Reaction".ELECTROANALYSIS 30.7(2018):1349-1356.
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