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Multi-Mode Full Spectrum Dark Field Microscope for Single Nanoparticle Localized Surface Plasmon Resonance Dynamics Study
Liu, L; Hao, YY; Deng, SH; Wang, K; Li, J; Wang, LH; Fan, CH; Li, JJ; Liu, HJ
2019
Source PublicationACTA PHYSICO-CHIMICA SINICA
ISSN1000-6818
Volume35Issue:4Pages:371—377
Subtype期刊论文
AbstractIn the last few decades, noble metal nanoparticles (MNP) have been widely used as imaging probes, in the field of bio-imaging, due to their localized surface plasmon resonance (LSPR) phenomenon. Compared to fluorescent probes, MNP imaging exhibits high sensitivity and outstanding signal-to-noise ratio, while the particle itself has good photostability; this makes the MNP probe the perfect candidate for long-term imaging. Currently the most popular MNP imaging and analysis method employs a dark-field microscope with a spectroscope. Since most dark-field microscopes use halogen lamp or mercury lamp as their illumination source, the illumination intensity and wavelength spectrum are limited. Both camera and spectroscopy require longer exposure time to collect sufficient scattering signal to generate a reasonable quality image and scattering spectrum. The narrow illumination spectrum also limits the size of the MNP that can be used (larger-diameter MNP tend to scatter in the near-infrared region). Therefore, a high-intensity and wide-spectrum illumination source is urgently needed in MNP imaging. In this study, we custom-designed a multi-mode dark field microscope by using a supercontinuum laser, comprising of a light-sheet illumination mode for wide-field imaging and a back focus mode for live spectrum analysis, as its illumination source. The total output of the supercontinuum laser was 2 W. Since it was a coherent illumination source it could be focused by the microscope objective to a near diffraction limit area for sufficient intensity. Moreover, since its wavelength spectrum was between 450 nm and 2200 nm, which covered most of the visible and near infrared region, it made the detection of the large-diameter MNP single particle possible. In the back-focus mode, the super-continuum laser first passed through an annular filter and then entered the objective from the microscope back port. In the lightsheet illumination mode, the laser was focused by a 400-mm cylindrical concave mirror to create a "sheet" and illuminate the sample from its side. In both the illumination modes, the illumination radiation was blocked from the camera to obtain the dark field illumination effect. By using a multi-mode dark field microscope, we could observe a 30-nm-diameter MNP single particle with a color CCD camera in its lightsheet illumination mode and a spectrum time resolution of 1 ms in its back-focus illumination mode. This custom-designed microscope could not only be used to study the MNP single particle in living cells, but more importantly, its application could also be potentially extended to all the MNP-probe-based cell imaging.
KeywordGOLD NANOPARTICLES SILVER NANOPARTICLES DNA TRANSPORT CELLS OLIGONUCLEOTIDE NANOSTRUCTURES HYBRIDIZATION MANIPULATION SCATTERING
DOI10.3866/PKU.WHXB201805022
Indexed BySCI
Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/31716
Collection中科院上海应用物理研究所2011-2020年
Affiliation1.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China;
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Liu, L,Hao, YY,Deng, SH,et al. Multi-Mode Full Spectrum Dark Field Microscope for Single Nanoparticle Localized Surface Plasmon Resonance Dynamics Study[J]. ACTA PHYSICO-CHIMICA SINICA,2019,35(4):371—377.
APA Liu, L.,Hao, YY.,Deng, SH.,Wang, K.,Li, J.,...&Liu, HJ.(2019).Multi-Mode Full Spectrum Dark Field Microscope for Single Nanoparticle Localized Surface Plasmon Resonance Dynamics Study.ACTA PHYSICO-CHIMICA SINICA,35(4),371—377.
MLA Liu, L,et al."Multi-Mode Full Spectrum Dark Field Microscope for Single Nanoparticle Localized Surface Plasmon Resonance Dynamics Study".ACTA PHYSICO-CHIMICA SINICA 35.4(2019):371—377.
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