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Substrate Metabolism-Driven Assembly of High-Quality CdSxSe1-x Quantum Dots in Escherichia coli: Molecular Mechanisms and Bioimaging Application
Tian, LJ; Min, Y; Li, WW; Chen, JJ; Zhou, NQ; Zhu, TT; Li, DB; Ma, JY; An, PF; Zheng, LR; Huang, H; Liu, YZ; Yu, HQ
2019
Source PublicationACS NANO
ISSN1936-0851
Volume13Issue:5Pages:5841—5851
Subtype期刊论文
AbstractBiosynthesis offers opportunities for cost-effective and sustainable production of semiconductor quantum dots (QDs), but is currently restricted by poor controllability on the synthesis process, resulting from limited knowledge on the assembly mechanisms and the lack of effective control strategies. In this work, we provide molecular-level insights into the formation mechanism of biogenic QDs (Bio-QDs) and its connection with the cellular substrate metabolism in Escherichia coli. Strengthening the substrate metabolism for producing more reducing power was found to stimulate the production of several reduced thiol-containing proteins (including glutaredoxin and thioredoxin) that play key roles in Bio-QDs assembly. This effectively diverted the transformation route of the selenium (Se) and cadmium (Cd) metabolic from Cd-3(PO4)(2) formation to CdSxSe1-x QDs assembly, yielding fine-sized (2.0 +/- 0.4 nm), high-quality Bio-QDs with quantum yield (5.2%) and fluorescence lifetime (99.19 ns) far exceeding the existing counterparts. The underlying mechanisms of Bio-QDs crystallization and development were elucidated by density functional theory calculations and molecular dynamics simulation. The resulting Bio-QDs were successfully used for bioimaging of cancer cells and tumor tissue of mice without extra modification. Our work provides fundamental knowledge on the Bio-QDs assembly mechanisms and proposes an effective, facile regulation strategy, which may inspire advances in controlled synthesis and practical applications of Bio-QDs as well as other bionanomaterials.
KeywordDYNAMICS SIMULATIONS OXIDATIVE STRESS NANOPARTICLES GLUTATHIONE BACTERIA BIOSYNTHESIS GLUTAREDOXINS INTEGRATION CADMIUM SYSTEM
DOI10.1021/acsnano.9b01581
Indexed BySCI
Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/31838
Collection中科院上海应用物理研究所2011-2020年
Affiliation1.Univ Sci & Technol China, Dept Appl Chem, CAS Key Lab Urban Pollutant Convers, Hefei 230026, Anhui, Peoples R China;
2.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201204, Peoples R China;
3.Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Lab, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Tian, LJ,Min, Y,Li, WW,et al. Substrate Metabolism-Driven Assembly of High-Quality CdSxSe1-x Quantum Dots in Escherichia coli: Molecular Mechanisms and Bioimaging Application[J]. ACS NANO,2019,13(5):5841—5851.
APA Tian, LJ.,Min, Y.,Li, WW.,Chen, JJ.,Zhou, NQ.,...&Yu, HQ.(2019).Substrate Metabolism-Driven Assembly of High-Quality CdSxSe1-x Quantum Dots in Escherichia coli: Molecular Mechanisms and Bioimaging Application.ACS NANO,13(5),5841—5851.
MLA Tian, LJ,et al."Substrate Metabolism-Driven Assembly of High-Quality CdSxSe1-x Quantum Dots in Escherichia coli: Molecular Mechanisms and Bioimaging Application".ACS NANO 13.5(2019):5841—5851.
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