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Thermococcus Eurythermalis Endonuclease IV Can Cleave Various Apurinic/Apyrimidinic Site Analogues in ssDNA and dsDNA
Wang, WW; Zhou, H; Xie, JJ; Yi, GS; He, JH; Wang, FP; Xiao, X; Liu, XP
2019
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN1422-0067
Volume20Issue:1Pages:
Subtype期刊论文
AbstractEndonuclease IV (EndoIV) is a DNA damage-specific endonuclease that mainly hydrolyzes the phosphodiester bond located at 5 of an apurinic/apyrimidinic (AP) site in DNA. EndoIV also possesses 3-exonuclease activity for removing 3-blocking groups and normal nucleotides. Here, we report that Thermococcus eurythermalis EndoIV (TeuendoIV) shows AP endonuclease and 3-exonuclease activities. The effect of AP site structures, positions and clustered patterns on the activity was characterized. The AP endonuclease activity of TeuendoIV can incise DNA 5 to various AP site analogues, including the alkane chain Spacer and polyethylene glycol Spacer. However, the short Spacer C2 strongly inhibits the AP endonuclease activity. The kinetic parameters also support its preference to various AP site analogues. In addition, the efficient cleavage at AP sites requires 2 normal nucleotides existing at the 5-terminus. The 3-exonuclease activity of TeuendoIV can remove one or more consecutive AP sites at the 3-terminus. Mutations on the residues for substrate recognition show that binding AP site-containing or complementary strand plays a key role for the hydrolysis of phosphodiester bonds. Our results provide a comprehensive biochemical characterization of the cleavage/removal of AP site analogues and some insight for repairing AP sites in hyperthermophile cells.
KeywordAPURINIC APYRIMIDINIC ENDONUCLEASE DOUBLE-STRANDED-DNA ESCHERICHIA-COLI NUCLEOTIDE INCISION EXONUCLEASE-III MAJOR HUMAN ABASIC SITES AP SITES REPAIR BINDING
DOI10.3390/ijms20010069
Indexed BySCI
Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/31871
Collection中科院上海应用物理研究所2011-2020年
Affiliation1.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, 800 Dong Chuan Rd, Shanghai 200240, Peoples R China;
2.Chinese Acad Sci, Shanghai Inst Appl Phys, 239 Zhangheng Rd, Shanghai 201204, Peoples R China;
3.Shanghai Jiao Tong Univ, Sch Naval Architecture Ocean & Civil Engn, State Key Lab Ocean Engn, 800 Dong Chuan Rd, Shanghai 200240, Peoples R China
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Wang, WW,Zhou, H,Xie, JJ,et al. Thermococcus Eurythermalis Endonuclease IV Can Cleave Various Apurinic/Apyrimidinic Site Analogues in ssDNA and dsDNA[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(1):—.
APA Wang, WW.,Zhou, H.,Xie, JJ.,Yi, GS.,He, JH.,...&Liu, XP.(2019).Thermococcus Eurythermalis Endonuclease IV Can Cleave Various Apurinic/Apyrimidinic Site Analogues in ssDNA and dsDNA.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(1),—.
MLA Wang, WW,et al."Thermococcus Eurythermalis Endonuclease IV Can Cleave Various Apurinic/Apyrimidinic Site Analogues in ssDNA and dsDNA".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.1(2019):—.
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