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Terminal deoxynucleotidyl transferase (TdT)-catalyzed homo-nucleotides-constituted ssDNA: Inducing tunable-size nanogap for core-shell plasmonic metal nanostructure and acting as Raman reporters for detection of Escherichia coli O157:H7
Zhou, YY; Fang, WN; Lai, KQ; Zhu, YH; Bian, XJ; Shen, JL; Li, Q; Wang, LH; Zhang, WJ; Yan, J
2019
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume141Pages:-
Subtype期刊论文
AbstractCore-shell plasmonic metal nanoparticles with interior nanogaps are superior nanostructures owing to their large signal enhancement for Surface enhanced Raman spectroscopy (SERS). Herein, we incorporated Terminal deoxynucleotidyl transferase (TdT)-catalyzed DNA in the preparation of core-shell nanostructures for the detection of Escherichia coli O157:H7 (E. coli O157:H7) cells. The elongated products-homo-nucleotides-composed of long single DNA strands (hn-D) are used not only to induce tunable-size nanogaps but also as Raman reporters with consistent and uniform signal enhancement. Using this synthetic process of hn-D-embedded core-shell nanoparticles (hn-DENPs), we found that the length of hn-D strands affects the size of the nanogap. In addition, performances of the specific Raman imaging of E. coli O157:H7, high detection sensitivity of 2 CFU/mL, and the recovery of 98.1%-105.2% measured in the real food samples, make hn-DENP a biosensor that will be widely used in biological detection.
KeywordOPTICAL-PROPERTIES NANOPARTICLES SERS DNA SPECTROSCOPY SCATTERING ENHANCEMENT GROWTH
DOI10.1016/j.bios.2019.111419
Indexed BySCI
Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/32237
Collection中科院上海应用物理研究所2011-2020年
Affiliation1.Shanghai Ocean Univ, Lab Qual & Safety Risk Assessment Aquat Prod Stor, Shanghai Engn Res Ctr Aquat Prod Proc & Preservat, Coll Food Sci & Technol,Minist Agr, Shanghai 201306, Peoples R China;
2.Chinese Acad Sci, Div Phys Biol & Bioimaging Ctr, Shanghai Synchrotron Radiat Facil, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China;
3.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China;
4.Fudan Univ, Peoples Hosp Shanghai 5, Shanghai 200032, Peoples R China;
5.Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R China;
6.Fudan Univ, State Key Lab Mol Engn, Shanghai 200032, Peoples R China
Recommended Citation
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Zhou, YY,Fang, WN,Lai, KQ,et al. Terminal deoxynucleotidyl transferase (TdT)-catalyzed homo-nucleotides-constituted ssDNA: Inducing tunable-size nanogap for core-shell plasmonic metal nanostructure and acting as Raman reporters for detection of Escherichia coli O157:H7[J]. BIOSENSORS & BIOELECTRONICS,2019,141:-.
APA Zhou, YY.,Fang, WN.,Lai, KQ.,Zhu, YH.,Bian, XJ.,...&Yan, J.(2019).Terminal deoxynucleotidyl transferase (TdT)-catalyzed homo-nucleotides-constituted ssDNA: Inducing tunable-size nanogap for core-shell plasmonic metal nanostructure and acting as Raman reporters for detection of Escherichia coli O157:H7.BIOSENSORS & BIOELECTRONICS,141,-.
MLA Zhou, YY,et al."Terminal deoxynucleotidyl transferase (TdT)-catalyzed homo-nucleotides-constituted ssDNA: Inducing tunable-size nanogap for core-shell plasmonic metal nanostructure and acting as Raman reporters for detection of Escherichia coli O157:H7".BIOSENSORS & BIOELECTRONICS 141(2019):-.
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