CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Ultrasensitive Electrochemical DNA Biosensor Based on a Label-Free Assembling Strategy Using a Triblock polyA DNA Probe
Wang, LL; Wen, YL; Yang, X; Xu, L; Liang, W; Zhu, Y; Wang, LH; Li, Y; Li, Y; Ding, M; Ren, SZ; Yang, ZZ; Lv, M; Zhang, JC; Ma, K; Liu, G
2019
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume91Issue:24Pages:16002-16009
Subtype期刊论文
AbstractMultiblock DNA probe attracted a large amount of scientific attention, for the development of multitarget biosensor and improved specificity/sensitivity. However, the development of multiblock DNA probes highly relied on the chemical synthesis of organic linkers or nanomaterials, which limited their practicability and biological compatibility. In this work, we developed a label-free assembling strategy using a triblock DNA capture probe, which connects two DNA probes with its intrinsic polyA fragment (probe-PolyA-probe, PAP). The middle polyA segment has a high affinity to the gold electrode surface, leading to excellent reproducibility, stability, and regeneration of our biosensor. Two flanking capture probes were tandemly co-assembled on the electrode surface with consistent spatial relationship and exactly the same amount. When combined with the target DNA, the hybridization stability was improved, because of the strong base stacking effect of two capture probes. The sensitivity of our biosensor was proved to be 10 fM, with a wide analysis range between 10 fM to 1 nM. Our PAP-based biosensor showed excellent specificity when facing mismatched DNA sequences. Even single nucleotide polymorphisms can be distinguished by each probe. The excellent practicability of our biosensor was demonstrated by analyzing genomic DNA both with and without PCR amplification.
KeywordSEQUENCE-SPECIFIC DETECTION SINGLE-STRANDED-DNA ESCHERICHIA-COLI FEMTOMOLAR DNA OLIGONUCLEOTIDE AMPLIFICATION POLYMERS DESIGN MIRNAS TOOL
DOI10.1021/acs.analchem.9b04757
Indexed BySCI
Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/32269
Collection中科院上海应用物理研究所2011-2020年
Affiliation1.Shanghai Inst Measurement & Testing Technol, Div Chem, Lab Biometrol, 1500 Zhang Heng Rd, Shanghai 201203, Peoples R China;
2.Natl Inst Metrol China, Div Chem Metrol & Analyt Sci, Beijing 102200, Peoples R China;
3.Chinese Acad Sci, Div Phys Biol & Bioimaging Ctr, CAS Key Lab Interfacial Phys & Technol, Shanghai Inst Appl Phys,Shanghai Synchrotron Radi, Shanghai 201800, Peoples R China;
4.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai 201210, Peoples R China
Recommended Citation
GB/T 7714
Wang, LL,Wen, YL,Yang, X,et al. Ultrasensitive Electrochemical DNA Biosensor Based on a Label-Free Assembling Strategy Using a Triblock polyA DNA Probe[J]. ANALYTICAL CHEMISTRY,2019,91(24):16002-16009.
APA Wang, LL.,Wen, YL.,Yang, X.,Xu, L.,Liang, W.,...&Liu, G.(2019).Ultrasensitive Electrochemical DNA Biosensor Based on a Label-Free Assembling Strategy Using a Triblock polyA DNA Probe.ANALYTICAL CHEMISTRY,91(24),16002-16009.
MLA Wang, LL,et al."Ultrasensitive Electrochemical DNA Biosensor Based on a Label-Free Assembling Strategy Using a Triblock polyA DNA Probe".ANALYTICAL CHEMISTRY 91.24(2019):16002-16009.
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