CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Visualizing mRNA in live mammalian cells
Wang, ZJ; Liu, WH; Fan, CH; Chen, N
2019
Source PublicationMETHODS
ISSN1046-2023
Volume161Pages:16-23
Subtype期刊论文
AbstractmRNAs play essential role in gene expression and regulation. Direct visualization of specific intracellular mRNAs can provide key information about mRNA distribution and dynamics that accelerating study of their functionality. This highlights the necessity of characterizing mRNA in mammalian cells with good spatial and temporal resolution. Live cell imaging and tracking of mRNA remains a challenge due to their relatively short half-life and lack of versatile imaging probes. This review provides an overview of current methods of live-cell mRNA imaging, including exogenous hybridization probes and genetically encoded strategies. It then focuses on the development and application of aptamer initiated fluorescence complementation (AiFC) method for real-time imaging of mammalian mRNAs and includes a detailed protocol for observing 13-actin mRNA in HeLa cells.
KeywordLIVING CELLS SPLICE VARIANTS IMAGING RNA IN-VITRO APTAMER FLUORESCENCE DYNAMICS TRANSLATION TRANSCRIPTS LOCALIZATION
DOI10.1016/j.ymeth.2019.03.008
Indexed BySCI
Language英语
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/32274
Collection中科院上海应用物理研究所2011-2019年
Affiliation1.Shanghai Normal Univ, Dept Chem, Shanghai 200234, Peoples R China;
2.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Wang, ZJ,Liu, WH,Fan, CH,et al. Visualizing mRNA in live mammalian cells[J]. METHODS,2019,161:16-23.
APA Wang, ZJ,Liu, WH,Fan, CH,&Chen, N.(2019).Visualizing mRNA in live mammalian cells.METHODS,161,16-23.
MLA Wang, ZJ,et al."Visualizing mRNA in live mammalian cells".METHODS 161(2019):16-23.
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