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A Carbon-Based DNA Framework Nano-Bio Interface for Biosensing with High Sensitivity and a High Signal-to-Noise Ratio
Su, J; Liu, WH; Chen, SX; Deng, WP; Dou, YZ; Zhao, ZH; Li, JY; Li, ZH; Yin, H; Ding, XT; Song, SP
2020
Source PublicationACS SENSORS
ISSN2379-3694
Volume5Issue:12Pages:3979-3987
Subtype期刊论文
AbstractBiosensing interface based on screen-printed carbon electrodes (SPCE) has been widely used for electrochemical biosensors in the field of medical diagnostics, food safety, and environmental monitoring. Nevertheless, SPCE always has a rough surface, which is easy to result in the disorder of nucleic acid capture probes, the nonspecific adsorption of signaling probes, the steric hindrance of target binding, and decrease in the signal-to-noise ratio and sensitivity of biosensors. So far, it still remains extremely challenging to develop high-efficiency carbon-based biosensing interfaces, especially for DNA probe-based assembly and functionalization. In this paper, we first used a specific DNA framework, DNA tetrahedron to solve the defects of the carbon interface, improving the biosensing ability of SPCE. With covalent coupling, the DNA tetrahedron could be immobilized on the carbon surface. Biosensing probe sequences extending from the DNA tetrahedron can be changed for different target molecules. We demonstrated that the improved SPCE could be applied for the detection of a variety of bioactive molecules. Typically, we designed gap hybridization, aptamer "sandwich" and aptamer competition reduction strategy for the detection of miRNA-141, thrombin, and ATP, respectively. High signal-to-noise ratio, sensitivity, and specificity were obtained for all of these kinds. Especially, the DNA tetrahedron-modified SPCE can work well with serum samples. The carbon-based DNA framework nano-bio interface would expand the use of SPCE and make electrochemical biosensors more available and valuable in clinical diagnosis.
KeywordPROSTATE-SPECIFIC ANTIGEN ELECTROCHEMICAL BIOSENSORS ASCORBIC-ACID URIC-ACID CANCER HYBRIDIZATION NANOPARTICLES CONDUCTIVITY TEMPERATURE PERFORMANCE
DOI10.1021/acssensors.0c01745
Indexed BySCI
Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/32670
Collection中科院上海应用物理研究所2011-2020年
Affiliation1.Chinese Acad Sci, Shanghai Adv Res Inst, Zhangjiang Lab, Shanghai Synchrotron Radiat Facil, Shanghai 201210, Peoples R China
2.Shanghai Jiao Tong Univ, Sch Biomed Engn, Inst Personalized Med, State Key Lab Oncogenes & Related Genes, Shanghai 200030, Peoples R China
3.Chinse Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Nanjing Univ Chinese Med, Dept Spine, TCM Hosp, Wuxi 214071, Jiangsu, Peoples R China
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Su, J,Liu, WH,Chen, SX,et al. A Carbon-Based DNA Framework Nano-Bio Interface for Biosensing with High Sensitivity and a High Signal-to-Noise Ratio[J]. ACS SENSORS,2020,5(12):3979-3987.
APA Su, J.,Liu, WH.,Chen, SX.,Deng, WP.,Dou, YZ.,...&Song, SP.(2020).A Carbon-Based DNA Framework Nano-Bio Interface for Biosensing with High Sensitivity and a High Signal-to-Noise Ratio.ACS SENSORS,5(12),3979-3987.
MLA Su, J,et al."A Carbon-Based DNA Framework Nano-Bio Interface for Biosensing with High Sensitivity and a High Signal-to-Noise Ratio".ACS SENSORS 5.12(2020):3979-3987.
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