CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
A novel sample delivery system based on circular motion for in situ serial synchrotron crystallography
Zhao, FZ; Sun, B; Yu, L; Xiao, QJ; Wang, ZJ; Chen, LL; Liang, H; Wang, QS; He, JH; Yin, DC
2020
Source PublicationLAB ON A CHIP
ISSN1473-0197
Volume20Issue:21Pages:3888-3898
Subtype期刊论文
AbstractA sample delivery system is one of the key parts of serial crystallography. It is the main limiting factor affecting the application of serial crystallography. At present, although a variety of useful sample delivery systems have been developed for serial crystallography, it still remains the focus of the field to further improve the performance and efficiency of sample delivery. In existing sample delivery technologies, samples are usually delivered in linear motion. Here we show that the samples can also be delivered using circular motion, which is a novel motion mode never tested before. In this paper, we report a microfluidic rotating-target sample delivery device, which is characterized by the circular motion of the samples, and verify the performance of the device at a synchrotron radiation facility. The microfluidic rotating-target sample delivery device consists of two parts: a microfluidic sample plate and a motion control system. Sample delivery is realized by rotating the microfluidic sample plate containing in situ grown crystals. This device offers significant advantages, including a very wide adjustable range of delivery speed, low background noise, and low sample consumption. Using the microfluidic rotating-target device, we carried out in situ serial crystallography experiments with lysozyme and proteinase K as model samples at the Shanghai Synchrotron Radiation Facility, and performed structural determination based on the serial crystallographic data. The results showed that the designed device is fully compatible with the synchrotron radiation facility, and the structure determination of proteins is successful using the serial crystallographic data obtained with the device.
KeywordPROTEIN-STRUCTURE DETERMINATION ROOM-TEMPERATURE FEMTOSECOND CRYSTALLOGRAPHY DATA-COLLECTION CHIP MICROFLUIDICS MICROCRYSTALS MEMBRANE PLATFORM LASERS
DOI10.1039/d0lc00443j
Indexed BySCI ; EI
Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/32684
Collection中科院上海应用物理研究所2011-2020年
Affiliation1.Northwestern Polytech Univ, Sch Life Sci, Xian, Peoples R China
2.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai, Peoples R China
4.Sichuan Univ, State Key Lab Biotherapy, Chengdu, Peoples R China
5.Wuhan Univ, Inst Adv Studies, Wuhan, Peoples R China
6.Northwestern Polytech Univ, Shenzhen Res Inst, Shenzhen, Peoples R China
Recommended Citation
GB/T 7714
Zhao, FZ,Sun, B,Yu, L,et al. A novel sample delivery system based on circular motion for in situ serial synchrotron crystallography[J]. LAB ON A CHIP,2020,20(21):3888-3898.
APA Zhao, FZ.,Sun, B.,Yu, L.,Xiao, QJ.,Wang, ZJ.,...&Yin, DC.(2020).A novel sample delivery system based on circular motion for in situ serial synchrotron crystallography.LAB ON A CHIP,20(21),3888-3898.
MLA Zhao, FZ,et al."A novel sample delivery system based on circular motion for in situ serial synchrotron crystallography".LAB ON A CHIP 20.21(2020):3888-3898.
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