CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Capturing transient antibody conformations with DNA origami epitopes
Zhang, P; Liu, XG; Liu, P; Wang, F; Ariyama, H; Ando, T; Lin, JP; Wang, LH; Hu, J; Li, B; Fan, CH
AbstractRevealing antibody-antigen interactions at the single-molecule level will deepen our understanding of immunology. However, structural determination under crystal or cryogenic conditions does not provide temporal resolution for resolving transient, physiologically or pathologically relevant functional antibody-antigen complexes. Here, we develop a triangular DNA origami framework with site-specifically anchored and spatially organized artificial epitopes to capture transient conformations of immunoglobulin Gs (IgGs) at room temperature. The DNA origami epitopes (DOEs) allows programmed spatial distribution of epitope spikes, which enables direct imaging of functional complexes with atomic force microscopy (AFM). We establish the critical dependence of the IgG avidity on the lateral distance of epitopes within 3-20nm at the single-molecule level. High-speed AFM imaging of transient conformations further provides structural and dynamic evidence for the IgG avidity from monovalent to bivalent in a single event, which sheds light on various applications including virus neutralization, diagnostic detection and cancer immunotherapy. Understanding antibody-antigen interactions is important to deepen the understanding of immunology. Here, the authors report on the application of DNA origami structures for the controlled presentation of antigens to study antibody binding behaviours at room temperature.
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Affiliation1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China
2.Frontiers Sci Ctr Transformat Mol, Inst Translat Med, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China
3.Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Haihe Educ Pk,38 Tongyan Rd, Tianjin 300353, Peoples R China
4.Nankai Univ, Tianjin Key Lab Mol Drug Res, Haihe Educ Pk,38 Tongyan Rd, Tianjin 300353, Peoples R China
5.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Biodesign Ctr, Tianjin 300308, Peoples R China
6.Southern Med Univ, Fengxian Hosp, Shanghai Jiao Tong Univ Affiliated Peoples Hosp 6, Joint Res Ctr Precis Med, South Campus, Shanghai 201499, Peoples R China
7.Kanazawa Univ, Nano Life Sci Inst WPI NanoLSI, Kanazawa, Ishikawa 9201192, Japan
8.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai Synchrotron Radiat Ctr, Shanghai 201210, Peoples R China
9.East China Normal Univ, Sch Chem & Mol Engn, Shanghai Key Lab Green Chem & Chem Proc, 500 Dongchuan Rd, Shanghai 200241, Peoples R China
10.Shanghai Jiao Tong Univ, Sch Med, Shanghai Key Lab Nucle Acids Chem & Nanomed, Renji Hosp,Inst Mol Med, Shanghai 200127, Peoples R China
Recommended Citation
GB/T 7714
Zhang, P,Liu, XG,Liu, P,et al. Capturing transient antibody conformations with DNA origami epitopes[J]. NATURE COMMUNICATIONS,2020,11(1):-.
APA Zhang, P.,Liu, XG.,Liu, P.,Wang, F.,Ariyama, H.,...&Fan, CH.(2020).Capturing transient antibody conformations with DNA origami epitopes.NATURE COMMUNICATIONS,11(1),-.
MLA Zhang, P,et al."Capturing transient antibody conformations with DNA origami epitopes".NATURE COMMUNICATIONS 11.1(2020):-.
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