CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Classifying Cell Types with DNA-Encoded Ligand-Receptor Interactions on the Cell Membrane
Zhang, LH; Wang, F; Li, Q; Wang, LH; Fan, CH; Li, J; Zhu, Y
2020
Source PublicationNANO LETTERS
ISSN1530-6984
Volume20Issue:5Pages:3521-3527
Subtype期刊论文
AbstractClustering, endocytosis, and intracellular transport of molecules on the cell membrane are critically dependent on the type of cells. However, the membrane-associated redistribution of molecules has not been exploited to realize cell classification for diagnostic purposes. Here, we develop a set of DNA-encoded artificial receptors and ligands to monitor the cell membrane redistribution. In this system, a cholesterol-modified single-stranded DNA strand serves as the receptor localized on the membrane, and a tetrahedral DNA framework (TDF) nanostructure with a complementary overhang serves as the ligand. The DNA-encoded receptor-ligand interaction is highly orthogonal, mimicking the dynamics of natural receptors and ligands on cells. We demonstrate that the dynamics of membrane redistribution can be resolved by the dual-color fluorescent patterns of the receptor-ligand interactions in a single image, which can be exploited to classify cell lines with high fidelity. This DNA-encoded method thus holds great promise for cell typing and diagnosis.
KeywordCANCER-CELLS INTERNALIZATION RECOGNITION PATHWAYS DELIVERY REVEALS DYNAMIN PROBES
DOI10.1021/acs.nanolett.0c00445
Indexed BySCI
Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/32745
Collection中科院上海应用物理研究所2011-2020年
Affiliation1.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, CAS Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China
2.Chinese Acad Sci, Bioimaging Ctr, Shanghai Adv Res Inst, Zhangjiang Lab,Shanghai Synchrotron Radiat Facil, Shanghai 201204, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Sch Chem & Chem Engn,Inst Mol Med,Frontiers Sci C, Shanghai 200024, Peoples R China
5.Chinese Acad Sci, Shanghai Adv Res Inst, Zhangjiang Lab, Shanghai 201204, Peoples R China
Recommended Citation
GB/T 7714
Zhang, LH,Wang, F,Li, Q,et al. Classifying Cell Types with DNA-Encoded Ligand-Receptor Interactions on the Cell Membrane[J]. NANO LETTERS,2020,20(5):3521-3527.
APA Zhang, LH.,Wang, F.,Li, Q.,Wang, LH.,Fan, CH.,...&Zhu, Y.(2020).Classifying Cell Types with DNA-Encoded Ligand-Receptor Interactions on the Cell Membrane.NANO LETTERS,20(5),3521-3527.
MLA Zhang, LH,et al."Classifying Cell Types with DNA-Encoded Ligand-Receptor Interactions on the Cell Membrane".NANO LETTERS 20.5(2020):3521-3527.
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