CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Direct DNA Methylation Profiling with an Electric Biosensor
Ban, DK; Liu, YS; Wang, ZJ; Ramachandran, S; Sarkar, N; Shi, Z; Liu, WH; Karkisaval, AG; Martinez-Loran, E; Zhang, F; Glinsky, G; Bandaru, PR; Fan, CH; Lal, R
2020
Source PublicationACS NANO
ISSN1936-0851
Volume14Issue:6Pages:6743-6751
Subtype期刊论文
AbstractDNA methylation is one of the principal epigenetic mechanisms that control gene expression in humans, and its profiling provides critical information about health and disease. Current profiling methods require chemical modification of bases followed by sequencing, which is expensive and time-consuming. Here, we report a direct and rapid determination of DNA methylation using an electric biosensor. The device consists of a DNA-tweezer probe integrated on a graphene field-effect transistor for label-free, highly sensitive, and specific methylation profiling. The device performance was evaluated with a target DNA that harbors a sequence of the methylguanine-DNA methyltransferase, a promoter of glioblastoma multiforme, a lethal brain tumor. The results show that we successfully profiled the methylated and nonmethylated forms at picomolar concentrations. Further, fluorescence kinetics and molecular dynamics simulations revealed that the position of the methylation site(s), their proximity, and accessibility to the toe-hold region of the tweezer probe are the primary determinants of the device performance.
KeywordLABEL-FREE DETECTION CYTOSINE METHYLATION GRAPHENE PROTEIN ASSAY HYBRIDIZATION DIAGNOSTICS DYNAMICS NANORODS DISEASE
DOI10.1021/acsnano.9b10085
Indexed BySCI
Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/32792
Collection中科院上海应用物理研究所2011-2020年
Affiliation1.Univ Calif San Diego, Inst Engn Med, La Jolla, CA 92093 USA
2.Univ Calif San Diego, Dept Mech & Aerosp Engn & Mat Sci & Engn, La Jolla, CA 92093 USA
3.Univ Calif San Diego, Dept Nanoengn, La Jolla, CA 92093 USA
4.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China
5.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Sch Chem & Chem Engn,Frontiers Sci Ctr Transforma, Shanghai 200240, Peoples R China
6.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Inst Mol Med, Shanghai 200240, Peoples R China
7.Univ Calif San Diego, Dept Mech & Aerosp Engn, Dept Bioengn Mat Sci & Engn, La Jolla, CA 92093 USA
8.Univ Calif San Diego, Dept Mech & Aerosp Engn, La Jolla, CA 92093 USA
9.Inner Mongolia Agr Univ, Sch Life Sci, Hohhot 010018, Peoples R China
10.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA (1
11.Univ Calif San Diego, Mat Sci & Engn, La Jolla, CA 92093 USA
12.Guangzhou Med Univ, Sch Basic Med Sci, Dept Biomed Engn,Guangzhou Inst Oral Dis, State Key Lab Resp Dis,Key Lab Oral Med,Stomatol, Guangzhou 511436, Peoples R China
Recommended Citation
GB/T 7714
Ban, DK,Liu, YS,Wang, ZJ,et al. Direct DNA Methylation Profiling with an Electric Biosensor[J]. ACS NANO,2020,14(6):6743-6751.
APA Ban, DK.,Liu, YS.,Wang, ZJ.,Ramachandran, S.,Sarkar, N.,...&Lal, R.(2020).Direct DNA Methylation Profiling with an Electric Biosensor.ACS NANO,14(6),6743-6751.
MLA Ban, DK,et al."Direct DNA Methylation Profiling with an Electric Biosensor".ACS NANO 14.6(2020):6743-6751.
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