CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
DNA Framework-Mediated Electrochemical Biosensing Platform for Amplification-Free MicroRNA Analysis
Wen, YL; Li, LY; Li, J; Lin, MH; Liu, G; Liang, W; Xu, L; Li, Y; Zuo, XL; Ren, SZ; Zhu, Y
2020
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume92Issue:6Pages:4498-4503
Subtype期刊论文
AbstractMicroRNAs (miRNAs) have been explored as biomarkers for early diagnosis of diseases like cancers. However, it remains challenging to detect low-level miRNAs in the total RNA from real samples in a facile approach. In this work, we report a two-phase miRNA biosensing strategy based on a modular framework nucleic acid (FNA) platform, which combines the high efficiency of homogeneous reaction and the convenience of heterogeneous biosensing. In the first phase, free DNA probes bind target miRNAs in a homogeneous solution, forming a DNA-RNA complex with high base stacking energy. Then, at the second phase, the universal FNA interface on the electrode selectively mediated the transition of the complex from the solution onto the interface for electrochemical signal generating and transduction. By applying this method, we detected as few as 1 aM of miR-141, a cancer marker miRNA, without the need for nucleic acid amplification. The dynamic range spans 10 orders of magnitude. We demonstrate multiplex miRNA detection and discrimination of highly homologous miRNAs with mismatches as few as a single base. We also show that this system can detect miR-141 in only 50 ng of total RNA samples from real cells, which allows discrimination of prostate cancer cells with normal cells. We envision this platform may satisfy the need for facile and high-throughput screening of early cancer markers.
DOI10.1021/acs.analchem.9b05616
Indexed BySCI
Language英语
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/32805
Collection中科院上海应用物理研究所2011-2020年
Affiliation1.Chinese Acad Sci, Shanghai Inst Appl Phys, Lab Phys Biol, Shanghai 201800, Peoples R China
2.Chinese Acad Sci, Shanghai Adv Res Inst, Zhangjiang Lab, Shanghai Synchrotron Radiat Facil, Shanghai 201204, Peoples R China
3.Shanghai Inst Measurement & Testing Technol, Div Chem & Ionizing Radiat Measurement Technol, Shanghai 201203, Peoples R China
4.Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Inst Mol Med, Shanghai 200240, Peoples R China
5.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China
Recommended Citation
GB/T 7714
Wen, YL,Li, LY,Li, J,et al. DNA Framework-Mediated Electrochemical Biosensing Platform for Amplification-Free MicroRNA Analysis[J]. ANALYTICAL CHEMISTRY,2020,92(6):4498-4503.
APA Wen, YL.,Li, LY.,Li, J.,Lin, MH.,Liu, G.,...&Zhu, Y.(2020).DNA Framework-Mediated Electrochemical Biosensing Platform for Amplification-Free MicroRNA Analysis.ANALYTICAL CHEMISTRY,92(6),4498-4503.
MLA Wen, YL,et al."DNA Framework-Mediated Electrochemical Biosensing Platform for Amplification-Free MicroRNA Analysis".ANALYTICAL CHEMISTRY 92.6(2020):4498-4503.
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