CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
DNA Framework-Supported Electrochemical Analysis of DNA Methylation for Prostate Cancers
Chen, SX; Su, J; Zhao, ZH; Shao, Y; Dou, YZ; Li, FW; Deng, WP; Shi, JY; Li, Q; Zuo, XL; Song, SP; Fan, CH
2020
Source PublicationNANO LETTERS
ISSN1530-6984
Volume20Issue:10Pages:7028-7035
Subtype期刊论文
AbstractEpigenetic alterations hold great promise as biomarkers for early stage cancer diagnosis. Nevertheless, direct identification of rare methylated DNA in the genome remains challenging. Here, we report an ultrasensitive framework nucleic acid-based electrochemical sensor for quantitative and highly selective analysis of DNA methylation. Notably, we can detect 160 fg of methylated DNA in million-fold unmethylated DNA samples using this electrochemical methylation-specific polymerase chain reaction (E-MSP) method. The high sensitivity of E-MSP enables one-step detection of low-abundance methylation at two different genes in patient serum samples. By using a combination test with two methylation alterations, we achieve high accuracy and sensitivity for reliable differentiation of prostate cancer and benign prostate hypertrophy (BPH). This new method sheds new light on translational use in early cancer diagnosis and in monitoring patients' responses to therapeutic agents.
DOI10.1021/acs.nanolett.0c01898
Indexed BySCI
Language英语
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/32806
Collection中科院上海应用物理研究所2011-2020年
Affiliation1.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, Shanghai 200050, Peoples R China
3.Shanghai Jiao Tong Univ, Sch Biomed Engn, Inst Personalized Med, Shanghai 200030, Peoples R China
4.Shanghai Jiao Tong Univ, Ruijin Hosp North, Sch Med, Dept Urol, Shanghai 201801, Peoples R China
5.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai Synchrotron Radiat Facil, Shanghai 201210, Peoples R China
6.Shanghai Jiao Tong Univ, Frontiers Sci Ctr Transformat Mol, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China
7.Shanghai Jiao Tong Univ, Inst Mol Med, Shanghai Key Lab Nucle Acids Chem & Nanomed, Renji Hosp,Sch Med, Shanghai 200240, Peoples R China
Recommended Citation
GB/T 7714
Chen, SX,Su, J,Zhao, ZH,et al. DNA Framework-Supported Electrochemical Analysis of DNA Methylation for Prostate Cancers[J]. NANO LETTERS,2020,20(10):7028-7035.
APA Chen, SX.,Su, J.,Zhao, ZH.,Shao, Y.,Dou, YZ.,...&Fan, CH.(2020).DNA Framework-Supported Electrochemical Analysis of DNA Methylation for Prostate Cancers.NANO LETTERS,20(10),7028-7035.
MLA Chen, SX,et al."DNA Framework-Supported Electrochemical Analysis of DNA Methylation for Prostate Cancers".NANO LETTERS 20.10(2020):7028-7035.
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