CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Encoding quantized fluorescence states with fractal DNA frameworks
Li, J; Dai, JB; Jiang, SX; Xie, M; Zhai, TT; Guo, LJ; Cao, ST; Xing, S; Qu, ZB; Zhao, Y; Wang, F; Yang, Y; Liu, L; Zuo, XL; Wang, LH; Yan, H; Fan, CH
AbstractSignal amplification in biological systems is achieved by cooperatively recruiting multiple copies of regulatory biomolecules. Nevertheless, the multiplexing capability of artificial fluorescent amplifiers is limited due to the size limit and lack of modularity. Here, we develop Cayley tree-like fractal DNA frameworks to topologically encode the fluorescence states for multiplexed detection of low-abundance targets. Taking advantage of the self-similar topology of Cayley tree, we use only 16 DNA strands to construct n-node (n=53) structures of up to 5 megadalton. The high level of degeneracy allows encoding 36 colours with 7 nodes by site-specifically anchoring of distinct fluorophores onto a structure. The fractal topology minimises fluorescence crosstalk and allows quantitative decoding of quantized fluorescence states. We demonstrate a spectrum of rigid-yet-flexible super-multiplex structures for encoded fluorescence detection of single-molecule recognition events and multiplexed discrimination of living cells. Thus, the topological engineering approach enriches the toolbox for high-throughput cell imaging. Though DNA framework-based scaffolds for biomolecular assembly are attractive for bioimaging applications, realizing super-multiplex fluorescent amplifiers remains a challenge. Here, the authors report a topological engineering approach to designing fractal DNA frameworks for multiplexed amplifiers.
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Affiliation1.Chinese Acad Sci, Bioimaging Ctr, Shanghai Adv Res Inst, Shanghai Synchrotron Radiat Facil,Zhangjiang Lab, Shanghai 201204, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, CAS Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China
3.Arizona State Univ, Ctr Mol Design & Biomimet, Sch Mol Sci, Biodesign Inst, Tempe, AZ 85287 USA
4.Shanghai Jiao Tong Univ, Renji Hosp, Frontiers Sci Ctr Transformat Mol, Sch Chem & Chem Engn,Inst Mol Med,Sch Med, Shanghai 200024, Peoples R China
5.East China Normal Univ, Sch Chem & Mol Engn, Shanghai Key Lab Green Chem & Chem Proc, 500 Dongchuan Rd, Shanghai 200241, Peoples R China
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Li, J,Dai, JB,Jiang, SX,et al. Encoding quantized fluorescence states with fractal DNA frameworks[J]. NATURE COMMUNICATIONS,2020,11(1):-.
APA Li, J.,Dai, JB.,Jiang, SX.,Xie, M.,Zhai, TT.,...&Fan, CH.(2020).Encoding quantized fluorescence states with fractal DNA frameworks.NATURE COMMUNICATIONS,11(1),-.
MLA Li, J,et al."Encoding quantized fluorescence states with fractal DNA frameworks".NATURE COMMUNICATIONS 11.1(2020):-.
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