CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Label-Free and Three-Dimensional Visualization Reveals the Dynamics of Plasma Membrane-Derived Extracellular Vesicles
Wang, KZ; Zhou, LM; Li, J; Liu, WJ; Wei, YH; Guo, Z; Fan, CH; Hu, J; Li, B; Wang, LH
2020
Source PublicationNANO LETTERS
ISSN1530-6984
Volume20Issue:9Pages:6313-6319
Subtype期刊论文
AbstractPlasma membrane-derived extracellular vesides (PEVs) are carriers of biological molecules that perform special cell cell communications. Nevertheless, the characterization of complicated PEV biology is hampered by the failure of current methods, mainly due to lack of specific labels and insufficient resolution. Here, we employed atomic force microscopy and scanning ion conductance microscopy, both capable of three-dimensional nanoscale resolution, for the label-free visualization of the PEV morphology, release, and uptake at the single-vesicle level. Except for classical microvesicles, we observed a cluster-like PEVs subtype in tumor cells. Moreover, both PEV subtype release times positively correlated with size. Through three-dimensional nanoscale imaging, we visualized the multiform PEV cell interaction behaviors of individual vesicles, which was challenged in conventional PEV imaging. Finally, we developed single-cell manipulation strategies to induce micrometer-sized PEV generation. Collectively, these results revealed the heterogeneous morphology and dynamics of PEVs at the single vesicle level, which provided new insight into the PEV biology.
KeywordEXOSOMES SECRETION CELLS
DOI10.1021/acs.nanolett.0c01735
Indexed BySCI
Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/32970
Collection中科院上海应用物理研究所2011-2020年
Affiliation1.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Shanghai Adv Res Inst, Shanghai 201210, Peoples R China
4.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China
5.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Inst Mol Med, Shanghai 200240, Peoples R China
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Wang, KZ,Zhou, LM,Li, J,et al. Label-Free and Three-Dimensional Visualization Reveals the Dynamics of Plasma Membrane-Derived Extracellular Vesicles[J]. NANO LETTERS,2020,20(9):6313-6319.
APA Wang, KZ.,Zhou, LM.,Li, J.,Liu, WJ.,Wei, YH.,...&Wang, LH.(2020).Label-Free and Three-Dimensional Visualization Reveals the Dynamics of Plasma Membrane-Derived Extracellular Vesicles.NANO LETTERS,20(9),6313-6319.
MLA Wang, KZ,et al."Label-Free and Three-Dimensional Visualization Reveals the Dynamics of Plasma Membrane-Derived Extracellular Vesicles".NANO LETTERS 20.9(2020):6313-6319.
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