CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Microplates for Crystal Growth and in situ Data Collection at a Synchrotron Beamline
Liang, M; Wang, ZJ; Wu, H; Yu, L; Sun, B; Zhou, H; Yu, F; Wang, QS; He, JH
2020
Source PublicationCRYSTALS
ISSN2073-4352
Volume10Issue:9Pages:-
Subtype期刊论文
AbstractAn efficient data collection method is important for microcrystals, because microcrystals are sensitive to radiation damage. Moreover, microcrystals are difficult to harvest and locate owing to refraction effects from the surface of the liquid drop or optically invisible, owing to their small size. Collecting X-ray diffraction data directly from the crystallization devices to completely eliminate the crystal harvesting step is of particular interest. To address these needs, novel microplates combining crystal growth and data collection have been designed for efficient in situ data collection and fully tested at Shanghai Synchrotron Radiation Facility (SSRF) crystallography beamlines. The design of the novel microplates fully adapts the advantage of in situ technology. Thin Kapton membranes were selected to seal the microplate for crystal growth, the crystallization plates can support hanging drop and setting drop vapor diffusion crystallization experiments. Then, the microplate was fixed on a magnetic base and mounted on the goniometer head for in situ data collection. Automatic grid scanning was applied for crystal location with a Blu-Ice data collection system and then in situ data collection was performed. The microcrystals of lysozyme were selected as the testing samples for diffraction data collection using the novel microplates. The results show that this method can achieve comparable data quality to that of the traditional method using the nylon loop. In addition, our method can efficiently and diversely perform data acquisition experiments, and be especially suitable for solving structures of multiple crystals at room temperature or cryogenic temperature.
KeywordX-RAY-DIFFRACTION PROTEIN CRYSTALLIZATION CRYSTALLOGRAPHY CHIP SOFTWARE PLATFORM DROPLET
DOI10.3390/cryst10090798
Indexed BySCI
Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/33005
Collection中科院上海应用物理研究所2011-2020年
Affiliation1.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai Synchrotron Radiat Facil, Shanghai 201204, Peoples R China
4.Wuhan Univ, Inst Adv Studies, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Liang, M,Wang, ZJ,Wu, H,et al. Microplates for Crystal Growth and in situ Data Collection at a Synchrotron Beamline[J]. CRYSTALS,2020,10(9):-.
APA Liang, M.,Wang, ZJ.,Wu, H.,Yu, L.,Sun, B.,...&He, JH.(2020).Microplates for Crystal Growth and in situ Data Collection at a Synchrotron Beamline.CRYSTALS,10(9),-.
MLA Liang, M,et al."Microplates for Crystal Growth and in situ Data Collection at a Synchrotron Beamline".CRYSTALS 10.9(2020):-.
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