CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Probing Transient DNA Conformation Changes with an Intercalative Fluorescent Excimer
Chen, Bin1; Huang, Qiuling2,3; Qu, Zhibei4; Li, Cong4; Li, Qian4; Shi, Jiye2,3; Fan, Chunhai4; Wang, Lihua2,3,5; Zuo, Xiaolei1; Shen, Jianlei4; Li, Jiang2,3,5
2020
Source PublicationAngewandte Chemie - International Edition
ISSN1433-7851
Subtype期刊论文
AbstractVariation of DNA conformation is important in regulating gene expression and mediating drug-DNA interactions. However, directly probing transient DNA conformation changes is challenging owing to the dynamic nature of this process. We show a label-free fluorescence method to monitor transient DNA conformation changes in DNA structures with various lengths and shapes using a DNA intercalator, K21. K21 can form transient excimers on the surface of DNA; the ratiometric emission of monomer and excimer correlate to DNA transient conformation stability in numerous DNA structures, including i-motifs, G-quadruplex structures, and single nucleotide mutation at random position. We analyzed the conformation dynamics of a single plasmid before and after enzyme digestion with confocal fluorescence microscopy. This method provides a label-free fluorescence strategy to probe transient conformation changes of DNA structures and has potential in uncovering transient genomic processes in living cells. © 2020 Wiley-VCH GmbH
KeywordDNA Conformations Fluorescence Fluorescence microscopy Gene expression Confocal fluorescence microscopy Conformation change Conformation dynamics DNA conformations Drug DNA interactions Fluorescence method G quadruplex structure Single nucleotides
DOI10.1002/anie.202014466
Indexed ByEI
Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/33085
Collection中科院上海应用物理研究所2011-2020年
Affiliation1.Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acids Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai; 200127, China;
2.Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai; 201800, China;
3.University of Chinese Academy of Sciences, Beijing; 100049, China;
4.School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai; 200240, China;
5.Bioimaging Center, Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai; 201210, China
Recommended Citation
GB/T 7714
Chen, Bin,Huang, Qiuling,Qu, Zhibei,et al. Probing Transient DNA Conformation Changes with an Intercalative Fluorescent Excimer[J]. Angewandte Chemie - International Edition,2020.
APA Chen, Bin.,Huang, Qiuling.,Qu, Zhibei.,Li, Cong.,Li, Qian.,...&Li, Jiang.(2020).Probing Transient DNA Conformation Changes with an Intercalative Fluorescent Excimer.Angewandte Chemie - International Edition.
MLA Chen, Bin,et al."Probing Transient DNA Conformation Changes with an Intercalative Fluorescent Excimer".Angewandte Chemie - International Edition (2020).
Files in This Item: Download All
File Name/Size DocType Version Access License
Probing Transient DN(1814KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chen, Bin]'s Articles
[Huang, Qiuling]'s Articles
[Qu, Zhibei]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chen, Bin]'s Articles
[Huang, Qiuling]'s Articles
[Qu, Zhibei]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chen, Bin]'s Articles
[Huang, Qiuling]'s Articles
[Qu, Zhibei]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Probing Transient DNA Conformation Changes with an Intercalative Fluorescent Excimer.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.