CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Programming Biomimetically Confined Aptamers with DNA Frameworks
Mao, XH; Liu, MM; Yan, L; Deng, MY; Li, F; Li, M; Wang, F; Li, J; Wang, LH; Tian, Y; Fan, CH; Zuo, XL
Source PublicationACS NANO
AbstractActive sites of proteins are generally encapsulated within three-dimensional peptide scaffolds that provide the molecular-scale confinement microenvironment. Nevertheless, the ability to tune thermodynamic stability in biomimetic molecular confinement relies on the macromolecular crowding effect of lack of stoichiometry and reconfigurability. Here, we report a framework nucleic acid (FNA)-based strategy to increase thermodynamic stability of aptamers. We demonstrate that the molecular-scale confinement increases the thermodynamic stability of aptamers via facilitated folding kinetics, which is confirmed by the single-molecule FRET (smFRET). Unfavorable conformations of aptamers are restricted as revealed by the Monte Carlo simulation. The binding affinity of the DNA framework-confined aptamer is improved by similar to 3-fold. With a similar strategy we improve the catalytic activity of heminbinding aptamer. Our approach thus shows high potential for designing protein-mimicking DNA nanostructures with enhanced binding affinity and catalytic activity for biosensing and biomedical engineering.
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Affiliation1.Shanghai Jiao Tong Univ, Inst Mol Med, Shanghai Key Lab Nucle Acid Chem & Nanomed, Renji Hosp,Sch Med, Shanghai 200127, Peoples R China
2.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Frontiers Sci Ctr Transformat Mol, Inst Translat Med, Shanghai 200240, Peoples R China
3.East China Normal Univ, Shanghai Key Lab Green Chem & Chem Proc, Dept Chem, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Div Phys Biol, Shanghai 201800, Peoples R China
5.Chinese Acad Sci, Shanghai Synchrotron Radiat Facil, Bioimaging Ctr, Shanghai Adv Res Inst,Zhangjiang Lab, Shanghai 201210, Peoples R China
6.Southern Med Univ, Shanghai Jiao Tong Univ Affiliated Peoples Hosp 6, Affiliated Fengxian Hosp, Joint Res Ctr Precis Med, South Campus, Shanghai 201499, Peoples R China
Recommended Citation
GB/T 7714
Mao, XH,Liu, MM,Yan, L,et al. Programming Biomimetically Confined Aptamers with DNA Frameworks[J]. ACS NANO,2020,14(7):8776-8783.
APA Mao, XH.,Liu, MM.,Yan, L.,Deng, MY.,Li, F.,...&Zuo, XL.(2020).Programming Biomimetically Confined Aptamers with DNA Frameworks.ACS NANO,14(7),8776-8783.
MLA Mao, XH,et al."Programming Biomimetically Confined Aptamers with DNA Frameworks".ACS NANO 14.7(2020):8776-8783.
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