CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Programming bulk enzyme heterojunctions for biosensor development with tetrahedral DNA framework
Song, P; Shen, JW; Ye, DK; Dong, BJ; Wang, F; Pei, H; Wang, JB; Shi, JY; Wang, LH; Xue, W; Huang, YR; Huang, G; Zuo, XL; Fan, CH
2020
Source PublicationNATURE COMMUNICATIONS
ISSN2041-1723
Volume11Issue:1Pages:-
Subtype期刊论文
AbstractProtein-protein interactions are spatially regulated in living cells to realize high reaction efficiency, as seen in naturally existing electron-transfer chains. Nevertheless, arrangement of chemical/biochemical components at the artificial device interfaces does not possess the same level of control. Here we report a tetrahedral DNA framework-enabled bulk enzyme heterojunction (BEH) strategy to program the multi-enzyme catalytic cascade at the interface of electrochemical biosensors. The construction of interpenetrating network of BEH at the millimeter-scale electrode interface brings enzyme pairs within the critical coupling length (CCL) of similar to 10nm, which in turn greatly improve the overall catalytic cascade efficiency by similar to 10-fold. We demonstrate the BEH generality with a range of enzyme pairs for electrochemically detecting clinically relevant molecular targets. As a proof of concept, a BEH-based sarcosine sensor enables single-step detection of the metabolic biomarker of sarcosine with ultrasensitivity, which hold the potential for precision diagnosis of early-stage prostate cancer.
KeywordGLUCOSE-RESPONSIVE INSULIN PROSTATE-CANCER SARCOSINE CELLS NANOSTRUCTURES METABOLISM LIPOSOMES MECHANISM BIOMARKER MACHINES
DOI10.1038/s41467-020-14664-8
Indexed BySCI
Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/33089
Collection中科院上海应用物理研究所2011-2020年
Affiliation1.Shanghai Jiao Tong Univ, Inst Mol Med, Dept Urol, Dept Nucl Med,Renji Hosp,Sch Med, Shanghai 200127, Peoples R China
2.Shanghai Jiao Tong Univ, Frontiers Sci Ctr Transformat Mol, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China
3.East China Normal Univ, Shanghai Key Lab Regulatory Biol, Inst Biomed Sci, Sch Life Sci, Shanghai 200241, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Div Phys Biol, Shanghai 201800, Peoples R China
5.East China Normal Univ, Shanghai Key Lab Green Chem & Chem Proc, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China
6.Chinese Acad Sci, Shanghai Adv Res Inst, Zhangjiang Lab, Bioimaging Ctr,Shanghai Synchrotron Radiat Facil, Shanghai 201210, Peoples R China
Recommended Citation
GB/T 7714
Song, P,Shen, JW,Ye, DK,et al. Programming bulk enzyme heterojunctions for biosensor development with tetrahedral DNA framework[J]. NATURE COMMUNICATIONS,2020,11(1):-.
APA Song, P.,Shen, JW.,Ye, DK.,Dong, BJ.,Wang, F.,...&Fan, CH.(2020).Programming bulk enzyme heterojunctions for biosensor development with tetrahedral DNA framework.NATURE COMMUNICATIONS,11(1),-.
MLA Song, P,et al."Programming bulk enzyme heterojunctions for biosensor development with tetrahedral DNA framework".NATURE COMMUNICATIONS 11.1(2020):-.
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