CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Ultrafast DNA Sensors with DNA Framework-Bridged Hybridization Reactions
Li, FQ; Mao, XH; Li, F; Li, M; Shen, JL; Ge, ZL; Fan, CH; Zuo, XL
2020
Source PublicationJOURNAL OF THE AMERICAN CHEMICAL SOCIETY
ISSN0002-7863
Volume142Issue:22Pages:9975-9981
Subtype期刊论文
AbstractIntracellular DNA-based hybridization reactions generally occur under tension rather than in free states, which are spatiotemporally controlled in physiological conditions. However, how nanomechanical forces affect DNA hybridization efficiencies in in-vitro DNA assays, for example, biosensors or biochips, remains largely elusive. Here, we design DNA framework-based nanomechanical handles that can control the stretching states of DNA molecules. Using a pair of tetrahedral DNA framework (TDF) nanostructured handles, we develop bridge DNA sensors that can capture target DNA with ultrafast speed and high efficiency. We find that the rigid TDF handles bind two ends of a single-stranded DNA (ssDNA) and hold it in a stretched state, with an apparent stretching length comparable to its counterpart of double-stranded DNA (dsDNA) via atomic force microscopy measurement. The DNA stretching effect of ssDNA is then monitored using single-molecule fluorescence energy transfer (FRET), resulting in decreased FRET efficiency in the stretched ssDNA. By controlling the stretching state of ssDNA, we obtained significantly improved hybridization kinetics (within 1 min) and hybridization efficiency (similar to 98%) under the target concentration of 500 nM. The bridge DNA sensors demonstrated high sensitivity (1 fM), high specificity (single mismatch mutation discrimination), and high selectivity (suitable for the detection in serum and blood) under the target concentration of 10 nM. Controlling the stretching state of ssDNA shows great potential in biosensors, bioimaging, and biochips applications.
KeywordNUCLEIC-ACIDS SECONDARY STRUCTURE KINETICS NANOSTRUCTURES ORIGIN
DOI10.1021/jacs.9b13737
Indexed BySCI
Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/33237
Collection中科院上海应用物理研究所2011-2020年
Affiliation1.Shanghai Jiao Tong Univ, Sch Med, Inst Mol Med, Shanghai Key Lab Nucle Acid Chem & Nanomed,Renji, Shanghai 200127, Peoples R China
2.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Shanghai 200127, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol & Bioimaging Ctr,CAS Key Lab Interf, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Li, FQ,Mao, XH,Li, F,et al. Ultrafast DNA Sensors with DNA Framework-Bridged Hybridization Reactions[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2020,142(22):9975-9981.
APA Li, FQ.,Mao, XH.,Li, F.,Li, M.,Shen, JL.,...&Zuo, XL.(2020).Ultrafast DNA Sensors with DNA Framework-Bridged Hybridization Reactions.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,142(22),9975-9981.
MLA Li, FQ,et al."Ultrafast DNA Sensors with DNA Framework-Bridged Hybridization Reactions".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 142.22(2020):9975-9981.
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