CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Ultrasensitive aptamer-based protein assays based on one-dimensional core-shell nanozymes
Zhang, R; Lu, N; Zhang, JX; Yan, RH; Li, J; Wang, LH; Wang, N; Lv, M; Zhang, M
2020
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume150Pages:-
Subtype期刊论文
AbstractIn enzyme-based immunoassys, the use of natural enzyme has been remarkably restricted by the inconvenience in preparation and storage, especially for point-of-care testing. Nanozymes, which can mimic the functions of natural enzymes, have been regarded as promising alternatives due to their robust stability and convenience in fabrication. Here we fabricated one-dimensional Fe3O4@C core-shell nanostructures via a solvent-thermal method. Thus prepared nanocomposites showed excellent peroxidase-like activity, capable of catalyzing chromogenic substrates into colored products in the presence of H2O2. We then developed a nanozyme-linked aptamer sorbent assay (NLASA) in a sandwich format, in which the as-prepared Fe3O4@C nanowires were employed as catalytic labels for colorimetric detection by naked eyes. In the detection of platelet-derived growth factor BB (PDGF-BB), this assay reliably exhibited detection limits as low as 10 fM, with a working range from 10 fM to 100 nM. By incorporating G-quadruplex-hemin DNAzyme with Fe3O4@C nanowires, the detection limit could be further lowered to 50 aM. The detection limit of PDGF-BB in 50% human serum was 100 fM. This ultrasensitive, cost-effective and easy-to-operate sensing platform offers new opportunities for protein detection in clinical diagnosis.
KeywordPEROXIDASE-LIKE ACTIVITY COLORIMETRIC DETECTION MAGNETIC NANOPARTICLES GOLD NANOSTRUCTURES PLATFORM OXIDASE NANOSENSORS NANOSHEETS MIMETICS
DOI10.1016/j.bios.2019.111881
Indexed BySCI ; EI
Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/33242
Collection中科院上海应用物理研究所2011-2020年
Affiliation1.Shanghai Univ Engn Sci, Sch Mat Engn, Shanghai 201620, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Div Phys Biol, Shanghai 201800, Peoples R China
3.Chinese Acad Sci, Shanghai Adv Res Inst, Zhangjiang Lab, Shanghai Synchrotron Radiat Facil, Shanghai 201210, Peoples R China
4.Shanghai Univ Engn Sci, Coll Chem & Chem Engn, Shanghai 201620, Peoples R China
Recommended Citation
GB/T 7714
Zhang, R,Lu, N,Zhang, JX,et al. Ultrasensitive aptamer-based protein assays based on one-dimensional core-shell nanozymes[J]. BIOSENSORS & BIOELECTRONICS,2020,150:-.
APA Zhang, R.,Lu, N.,Zhang, JX.,Yan, RH.,Li, J.,...&Zhang, M.(2020).Ultrasensitive aptamer-based protein assays based on one-dimensional core-shell nanozymes.BIOSENSORS & BIOELECTRONICS,150,-.
MLA Zhang, R,et al."Ultrasensitive aptamer-based protein assays based on one-dimensional core-shell nanozymes".BIOSENSORS & BIOELECTRONICS 150(2020):-.
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