CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Virus Disinfection from Environmental Water Sources Using Living Engineered Biofilm Materials
Pu, JH; Liu, Y; Zhang, JC; An, BL; Li, YF; Wang, XY; Din, K; Qin, C; Li, K; Cui, MK; Liu, SY; Huang, YY; Wang, YY; Lv, Y; Huang, JD; Cui, ZQ; Zhao, SW; Zhong, C
2020
Source PublicationADVANCED SCIENCE
ISSN2198-3844
Volume7Issue:14Pages:-
Subtype期刊论文
AbstractWaterborne viruses frequently cause disease outbreaks and existing strategies to remove such viral pathogens often involve harsh or energy-consuming water treatment processes. Here, a simple, efficient, and environmentally friendly approach is reported to achieve highly selective disinfection of specific viruses with living engineered biofilm materials. As a proof-of-concept, Escherichia coli biofilm matrix protein CsgA was initially genetically fused with the influenza-virus-binding peptide (C5). The resultant engineered living biofilms could correspondingly capture virus particles directly from aqueous solutions, disinfecting samples to a level below the limit-of-detection for a qPCR-based detection assay. By exploiting the surface-adherence properties of biofilms, it is further shown that polypropylene filler materials colonized by the CsgA-C5 biofilms can be utilized to disinfect river water samples with influenza titers as high as 1 x 10(7) PFU L-1. Additionally, a suicide gene circuit is designed and applied in the engineered strain that strictly limits the growth of bacterial, therefore providing a viable route to reduce potential risks confronted with the use of genetically modified organisms. The study thus illustrates that engineered biofilms can be harvested for the disinfection of pathogens from environmental water samples in a controlled manner and highlights the unique biology-only properties of living substances for material applications.
KeywordBACTERIAL INACTIVATION PATHOGENS
DOI10.1002/advs.201903558
Indexed BySCI ; EI
Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/33264
Collection中科院上海应用物理研究所2011-2020年
Affiliation1.ShanghaiTech Univ, Sch Phys Sci & Technol, Mat & Phys Biol Div, Shanghai 201210, Peoples R China
2.ShanghaiTech Univ, iHuman Inst, Shanghai 201210, Peoples R China
3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
4.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China
5.Wuxi Biol Co Ltd, Suzhou 215100, Peoples R China
6.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
7.Chinese Acad Sci, Mat Synthet Biol Ctr, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China
8.Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, CAS Key Lab Quantitat Engn Biol, Shenzhen 518055, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Pu, JH,Liu, Y,Zhang, JC,et al. Virus Disinfection from Environmental Water Sources Using Living Engineered Biofilm Materials[J]. ADVANCED SCIENCE,2020,7(14):-.
APA Pu, JH.,Liu, Y.,Zhang, JC.,An, BL.,Li, YF.,...&Zhong, C.(2020).Virus Disinfection from Environmental Water Sources Using Living Engineered Biofilm Materials.ADVANCED SCIENCE,7(14),-.
MLA Pu, JH,et al."Virus Disinfection from Environmental Water Sources Using Living Engineered Biofilm Materials".ADVANCED SCIENCE 7.14(2020):-.
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