CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
DNA Structural Changes Under Different Stretching Methods Studied By Molecular Dynamics Simulations
Qi, WP; Lei, XL; Fang, HP; Lei, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, POB 800-204, Shanghai 201800, Peoples R China
2010
Source PublicationCHEMPHYSCHEM
ISSN1439-4235
Volume11Issue:10Pages:6
AbstractWe present a molecular dynamics simulation study of 22-mer DNA conformational variations obtained by stretching both 3'-termini and both 5'-termini. Stretching 3'-termini by 3.5 nm required 142 kJ mol(-1) and the force plateau was similar to 80 pN, whereas stretching 5'-termini by the same length required 190 kJ mol(-1) and the force plateau was similar to 100 pN. Stretching 3'-termini led to a larger untwisting of the double helix and the successive base pairs rolled to the side of the DNA minor groove, while stretching 5'-termini resulted in the base pairs rolling to the major groove side and reducing of the diameter of DNA molecule. The most distinctive difference between stretching 3'-termini and 5'-termini was that at the force plateau region stretching the 5'-termini resulted in breakage of the base pairs, which considerably disturbed the structure of the DNA double helix. All of the variations of base rotation and translation for both stretching methods took place when the relative length of DNA I was longer than 1.2, which was the point the force plateau appeared.
KeywordConformational Analysis Dna Genetic Engineering Molecular Biology Molecular Dynamics
Subject AreaChemistry ; Physics
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000280428400017
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/6890
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorLei, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, POB 800-204, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Qi, WP,Lei, XL,Fang, HP,et al. DNA Structural Changes Under Different Stretching Methods Studied By Molecular Dynamics Simulations[J]. CHEMPHYSCHEM,2010,11(10):6.
APA Qi, WP,Lei, XL,Fang, HP,&Lei, XL .(2010).DNA Structural Changes Under Different Stretching Methods Studied By Molecular Dynamics Simulations.CHEMPHYSCHEM,11(10),6.
MLA Qi, WP,et al."DNA Structural Changes Under Different Stretching Methods Studied By Molecular Dynamics Simulations".CHEMPHYSCHEM 11.10(2010):6.
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