CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Enzymatic Digestion of Single DNA Molecules Anchored on Nanogold-Modified Surfaces
Lu, JH(吕军鸿); Ye, M(叶鸣); Duan, N; Li, B(李宾); Li, B (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, POB 800-204, Shanghai 201800, Peoples R China
2009
Source PublicationNANOSCALE RESEARCH LETTERS
ISSN1931-7573
Volume4Issue:9Pages:6
AbstractTo study enzyme-DNA interactions at single molecular level, both the attachment points and the immediate surroundings of surfaces must be carefully considered such that they do not compromise the structural information and biological properties of the sample under investigation. The present work demonstrates the feasibility of enzymatic digestion of single DNA molecules attached to nanoparticle-modified surfaces. With Nanogold linking DNA to the mica surface by electrostatic interactions, advantageous conditions with fewer effects on the length and topography of DNA are obtained, and an appropriate environment for the activities of DNA is created. We demonstrate that by using Dip-Pen Nanolithography, individual DNA molecules attached to modified mica surfaces can be efficiently digested by DNase I.
KeywordGold Nanoparticles Mica Dna Atomic Force Microscopy Dip-pen Nanolithography
Subject AreaScience & Technology - Other Topics ; Materials Science ; Physics
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000269013100010
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/6911
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorLi, B (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, POB 800-204, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Lu, JH,Ye, M,Duan, N,et al. Enzymatic Digestion of Single DNA Molecules Anchored on Nanogold-Modified Surfaces[J]. NANOSCALE RESEARCH LETTERS,2009,4(9):6.
APA Lu, JH,Ye, M,Duan, N,Li, B,&Li, B .(2009).Enzymatic Digestion of Single DNA Molecules Anchored on Nanogold-Modified Surfaces.NANOSCALE RESEARCH LETTERS,4(9),6.
MLA Lu, JH,et al."Enzymatic Digestion of Single DNA Molecules Anchored on Nanogold-Modified Surfaces".NANOSCALE RESEARCH LETTERS 4.9(2009):6.
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