CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Adenosine detection by using gold nanoparticles and designed aptamer sequences
Li, F; Zhang, J; Cao, XN; Wang, LH(王丽华); Li, D(李迪); Song, SP(宋世平); Ye, BC; Fan, CH(樊春海); Wang, LH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
2009
Source PublicationANALYST
ISSN0003-2654
Volume134Issue:7Pages:1355
AbstractBased on gold nanoparticles (AuNPs) and engineered DNA aptamers, we designed a novel bioassay strategy for the detection of adenosine as a small target molecule. In this design, an aptamer is engineered to consist of two pieces of random-coil like ssDNA which are respectively attached to AuNPs through their 50-thiol-modified end. They can reassemble into the intact aptamer tertiary structure and induce nanoparticle aggregation in the presence of the specific target. Results have demonstrated that gold nanoparticles can effectively differentiate these two different DNA structures via their characteristic surface plasmon resonance-based color change. With this method, adenosine can be selectively detected in the low micromolar range, which means that the strategy reported here can be applicable to the detection of several other small target molecules.
Subject AreaChemistry
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000267499800014
Citation statistics
Cited Times:128[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/7641
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorWang, LH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Li, F,Zhang, J,Cao, XN,et al. Adenosine detection by using gold nanoparticles and designed aptamer sequences[J]. ANALYST,2009,134(7):1355.
APA Li, F.,Zhang, J.,Cao, XN.,Wang, LH.,Li, D.,...&Wang, LH .(2009).Adenosine detection by using gold nanoparticles and designed aptamer sequences.ANALYST,134(7),1355.
MLA Li, F,et al."Adenosine detection by using gold nanoparticles and designed aptamer sequences".ANALYST 134.7(2009):1355.
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