Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Adenosine detection by using gold nanoparticles and designed aptamer sequences | |
| Li, F; Zhang, J; Cao, XN; Wang, LH(王丽华）; Li, D（李迪）; Song, SP（宋世平）; Ye, BC; Fan, CH（樊春海）; Wang, LH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China | |
| 2009 | |
| Source Publication | ANALYST
 |
| ISSN | 0003-2654 |
| Volume | 134Issue:7Pages:1355 |
| Abstract | Based on gold nanoparticles (AuNPs) and engineered DNA aptamers, we designed a novel bioassay strategy for the detection of adenosine as a small target molecule. In this design, an aptamer is engineered to consist of two pieces of random-coil like ssDNA which are respectively attached to AuNPs through their 50-thiol-modified end. They can reassemble into the intact aptamer tertiary structure and induce nanoparticle aggregation in the presence of the specific target. Results have demonstrated that gold nanoparticles can effectively differentiate these two different DNA structures via their characteristic surface plasmon resonance-based color change. With this method, adenosine can be selectively detected in the low micromolar range, which means that the strategy reported here can be applicable to the detection of several other small target molecules. |
| Subject Area | Chemistry |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000267499800014 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/7641 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Wang, LH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| Recommended Citation GB/T 7714 | Li, F,Zhang, J,Cao, XN,et al. Adenosine detection by using gold nanoparticles and designed aptamer sequences[J]. ANALYST,2009,134(7):1355. |
| APA | Li, F.,Zhang, J.,Cao, XN.,Wang, LH.,Li, D.,...&Wang, LH .(2009).Adenosine detection by using gold nanoparticles and designed aptamer sequences.ANALYST,134(7),1355. |
| MLA | Li, F,et al."Adenosine detection by using gold nanoparticles and designed aptamer sequences".ANALYST 134.7(2009):1355. |
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