CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
An enzyme-based E-DNA sensor for sequence-specific detection of femtomolar DNA targets
Liu, G; Wan, Y; Gau, V; Zhang, J; Wang, LH(王丽华); Song, SP(宋世平); Fan, CH(樊春海); Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
2008
Source PublicationJOURNAL OF THE AMERICAN CHEMICAL SOCIETY
ISSN0002-7863
Volume130Issue:21Pages:6820
AbstractIn this work, we report an enzyme-based E-DNA sensor for the sequence-specific detection of nucleic acids. This DNA sensor employs a "stem-loop" DNA probe dually labeled with biotin and digoxigenin (DIG). The probe is immobilized at an avidin-modified electrode surface via the biotin-avidin bridge, and the DIG serves as an affinity tag for the enzyme binding. In the initial state of the sensor, the probe adopts the stem-loop structure, which shields DIG from being approached by a bulky horseradish peroxidase-linked-anti-DIG antibody (anti-DIG-HRP) due to the steric effect. After hybridization, the probe undergoes a significant conformational change, forcing DIG away from the electrode. As a result, the DIG label becomes accessible by the anti-DIG-HRP, and the target hybridization event can be sensitively transcluced via the enzymatically amplified electrochemical current signal. By using this new strategy, we demonstrate that the prototype E-DNA sensor has been able to detect as low as femtomolar DNA targets with excellent differentiation ability for even single mismatches.
Subject AreaNuclear Science & Technology ; Physics
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000256158200052
Citation statistics
Cited Times:365[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/7645
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorFan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Liu, G,Wan, Y,Gau, V,et al. An enzyme-based E-DNA sensor for sequence-specific detection of femtomolar DNA targets[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2008,130(21):6820.
APA Liu, G.,Wan, Y.,Gau, V.,Zhang, J.,Wang, LH.,...&Fan, CH .(2008).An enzyme-based E-DNA sensor for sequence-specific detection of femtomolar DNA targets.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,130(21),6820.
MLA Liu, G,et al."An enzyme-based E-DNA sensor for sequence-specific detection of femtomolar DNA targets".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 130.21(2008):6820.
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