Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Crystal structure and function of C-terminal Sau3AI domain | |
| Xu, CY（徐春艳）; Yu, F（郁峰）; Xu, SJ（许世杰）; Ding, Y; Sun, LH; Tang, L（唐琳）; Hu, XJ; Zhang, ZH; He, JH（何建华）; Hu, XJ (reprint author), Fudan Univ, Dept Phys & Biophys, Shanghai 200433, Peoples R China | |
| 2009 | |
| Source Publication | BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
 |
| ISSN | 1570-9639 |
| Volume | 1794Issue:1Pages:118 |
| Abstract | Sau3AI is a type 11 restriction enzyme that recognizes the 5'-GATC-3' sequence in double-strand DNA and cleaves at 5' to the G residue. The C-terminal domain of SaU3AI (Sau3AI-C), which contains amino acids from 233 to 489, was crystallized and its structure was solved by using the Multi-wavelength Anomalous Diffraction method. The Sau3AI-C structure at 1.9 angstrom resolution is similar to the structure of MutH, a DNA mismatch repair protein that shares high sequence similarity with the N-terminal Sau3AI domain. The functional analysis shows that Sau3AI-C can bind DNA with one recognition sequence but has no cleavage activity. These results indicate that Sau3AI is a pseudo-dimer belonging to the type IIe restriction enzymes and the Sau3AI-C is the allosteric effector domain that assists DNA binding and cleavage. (C) 2008 Elsevier B.V. All rights reserved. |
| Subject Area | Biochemistry & Molecular Biology ; Biophysics |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000261863300014 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/7680 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Hu, XJ (reprint author), Fudan Univ, Dept Phys & Biophys, Shanghai 200433, Peoples R China |
| Recommended Citation GB/T 7714 | Xu, CY,Yu, F,Xu, SJ,et al. Crystal structure and function of C-terminal Sau3AI domain[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,2009,1794(1):118. |
| APA | Xu, CY.,Yu, F.,Xu, SJ.,Ding, Y.,Sun, LH.,...&Hu, XJ .(2009).Crystal structure and function of C-terminal Sau3AI domain.BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,1794(1),118. |
| MLA | Xu, CY,et al."Crystal structure and function of C-terminal Sau3AI domain".BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 1794.1(2009):118. |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
| Crystal structure an（629KB） | 开放获取 | -- | View Download | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment