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Crystal structure and function of C-terminal Sau3AI domain
Xu, CY(徐春艳); Yu, F(郁峰); Xu, SJ(许世杰); Ding, Y; Sun, LH; Tang, L(唐琳); Hu, XJ; Zhang, ZH; He, JH(何建华); Hu, XJ (reprint author), Fudan Univ, Dept Phys & Biophys, Shanghai 200433, Peoples R China
2009
Source PublicationBIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
ISSN1570-9639
Volume1794Issue:1Pages:118
AbstractSau3AI is a type 11 restriction enzyme that recognizes the 5'-GATC-3' sequence in double-strand DNA and cleaves at 5' to the G residue. The C-terminal domain of SaU3AI (Sau3AI-C), which contains amino acids from 233 to 489, was crystallized and its structure was solved by using the Multi-wavelength Anomalous Diffraction method. The Sau3AI-C structure at 1.9 angstrom resolution is similar to the structure of MutH, a DNA mismatch repair protein that shares high sequence similarity with the N-terminal Sau3AI domain. The functional analysis shows that Sau3AI-C can bind DNA with one recognition sequence but has no cleavage activity. These results indicate that Sau3AI is a pseudo-dimer belonging to the type IIe restriction enzymes and the Sau3AI-C is the allosteric effector domain that assists DNA binding and cleavage. (C) 2008 Elsevier B.V. All rights reserved.
Subject AreaBiochemistry & Molecular Biology ; Biophysics
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000261863300014
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/7680
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorHu, XJ (reprint author), Fudan Univ, Dept Phys & Biophys, Shanghai 200433, Peoples R China
Recommended Citation
GB/T 7714
Xu, CY,Yu, F,Xu, SJ,et al. Crystal structure and function of C-terminal Sau3AI domain[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,2009,1794(1):118.
APA Xu, CY.,Yu, F.,Xu, SJ.,Ding, Y.,Sun, LH.,...&Hu, XJ .(2009).Crystal structure and function of C-terminal Sau3AI domain.BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,1794(1),118.
MLA Xu, CY,et al."Crystal structure and function of C-terminal Sau3AI domain".BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 1794.1(2009):118.
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