CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Cytotoxic and radiosensitizing effects of nano-C-60 on tumor cells in vitro
Ni, J; Wu, QY; Li, YG(李宇国); Guo, ZX; Tang, GS; Sun, D; Gao, F; Cai, JM; Cai, JM (reprint author), Mil Med Coll 2, Dept Radiat Med, Shanghai 200433, Peoples R China
2008
Source PublicationJOURNAL OF NANOPARTICLE RESEARCH
ISSN1388-0764
Volume10Issue:4Pages:643
AbstractThere is growing evidence in recent years that the pristine fullerene may be endowed with strong pro-oxidant capacity to biological samples. In this investigation we tested the hypothesis that water-soluble fullerene-C-60 (nano-C-60) may interact with ionizing radiation enhancing its antiproliferative effects. The two tumor cell lines with different radiosensitivity B16 and SMMU-7721 were treated by a combination of pristine fullerene and Co-60 gamma irradiation. We measured cell survival rates, apoptotic characteristics, reactive oxygen species (ROS) production and alteration of cell diameter with or without gamma-irradiation. There was reduced survival with B16 and SMMU-7721 cells exposed to nano-C-60, with the inhibitory concentrations reducing the viability by 50% to 65 part per billion (ppb) and 150 ppb respectively. For cells exposed to nano-C-60 prior to gamma-irradiation, damage to cell membranes and increased numbers of apoptotic cells were detected by morphologic Hoechst-staining analysis and Annexin V/propidium iodide double-staining. In cells exposed to nano-C-60, there were increased levels of ROS, as measured by fluorescence detection under laser confocal microscopy. Preincubation with non-toxic pristine C-60 before gamma-ray caused enlargement of cells with increased diameter. The results show that nano-C-60 inhibits the growth of tumor cells at certain concentrations and increases the effects of Co-60 gamma-irradiation, possibly through the elevated production of cellular ROS and the membrane disruption. Data in this study indicates a possible consideration of using C-60 as a candidate of sensitization modifier in tumor radiation biology.
Subject AreaSpectroscopy
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000253626900010
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/7682
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorCai, JM (reprint author), Mil Med Coll 2, Dept Radiat Med, Shanghai 200433, Peoples R China
Recommended Citation
GB/T 7714
Ni, J,Wu, QY,Li, YG,et al. Cytotoxic and radiosensitizing effects of nano-C-60 on tumor cells in vitro[J]. JOURNAL OF NANOPARTICLE RESEARCH,2008,10(4):643.
APA Ni, J.,Wu, QY.,Li, YG.,Guo, ZX.,Tang, GS.,...&Cai, JM .(2008).Cytotoxic and radiosensitizing effects of nano-C-60 on tumor cells in vitro.JOURNAL OF NANOPARTICLE RESEARCH,10(4),643.
MLA Ni, J,et al."Cytotoxic and radiosensitizing effects of nano-C-60 on tumor cells in vitro".JOURNAL OF NANOPARTICLE RESEARCH 10.4(2008):643.
Files in This Item: Download All
File Name/Size DocType Version Access License
Cytotoxic and radios(351KB) 开放获取--View Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ni, J]'s Articles
[Wu, QY]'s Articles
[Li, YG(李宇国)]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ni, J]'s Articles
[Wu, QY]'s Articles
[Li, YG(李宇国)]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ni, J]'s Articles
[Wu, QY]'s Articles
[Li, YG(李宇国)]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Cytotoxic and radiosensitizing effects of nano-C-60 on tumor cells in vitro.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.